›› 2007, Vol. 50 ›› Issue (5): 474-480.
• RESEARCH PAPERS • Previous Articles Next Articles
LUO Qian, FENG Xia, LU Li-Hua, WAN Shu-Qing, CHEN Huan-Yu
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Abstract: The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is an important insect pest in cruciferous vegetables and has developed high resistance to avermectin all over the world. In this study, AFLP was used to construct a linkage map of resistance to avermectin in diamondback moth. The mapping families consisted of the susceptible strain, the abamectin-resistant strain and their backcross progeny. A total of 1 044 DNA bands and 271 polymorphic loci were amplified using 10 combinations of AFLP primers. Chi-square (χ2) analysis indicated that only 123 bands fit the segregation ratio of 1∶1 (P=0.05) in these polymorphic loci. Twenty-eight linkage groups were constructed with 112 bands, covering about 1 222.7 cM of the genomics of the diamondback moth. Three markers of E1M4-15, E1M1-4, and E1M4-2, linked with abamectin-resistant gene were situated on the same linkage group and their distance apart from resistant gene was 0 cM, 8.3 cM, and 13.1 cM, respectively. Comparison with the constructed linkage map showed that the gene was located on the fifth linkage group. These AFLP markers associated with resistance to abamectin show a strong potential of application in monitoring diamondback moth resistance to abamectin.
Key words: Plutella xylostella, abamectin, resistance gene, AFLP marker
LUO Qian, FENG Xia, LU Li-Hua, WAN Shu-Qing, CHEN Huan-Yu. Linkage mapping of resistance to abamectin in the diamondback moth using AFLP marker[J]., 2007, 50(5): 474-480.
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http://www.insect.org.cn/EN/Y2007/V50/I5/474
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