东亚飞蝗中肠几丁质酶基因的克隆、序列分析及组织定位

›› 2007, Vol. 50 ›› Issue (6): 555-559.doi:

• RESEARCH PAPERS • Previous Articles Next Articles

Cloning, sequence analysis and tissue expression of chitinase gene from the midgut of Locusta migratoria manilensis (Meyen)

ZHANG Ba-Sheng, CAO Yue-Qing, ZHANG Wei, YIN You-Ping, WANG Zhong-Kang, PENG Guo-Xiong, XIA Yu-Xian

(Genetic Engineering Research Center, School of Bioengineering, Chongqing University, Chongqing Engineering Research Center for Fungal Insecticides, The Key Laboratory of Gene Function and Expression Regulation, Chongqing 400044, China)

Online:2007-06-20 Published:2007-12-20
Contact: XIA Yu-Xian

Abstract

Abstract: The full cDNA of chitinase gene (LmChi) from the midgut of Locusta migratoria manilensis (Meyen) was cloned by means of 3′-RACE and 5′-RACE. The results showed that the cDNA was 1 604 bp in length and contained an open reading frame (ORF) of 1 452 bp (GenBank accession number: EF092841), which encoded 483 amino acids. The deduced amino acid sequence, which was composed of a signal peptide, a chitinase active site, a C-terminal serine-rich region and a domain normal present in chitinase, showed a high similarity with other insect chitinases from family 18.Semi-quantitative RT-PCR indicated that LmChi expressed only in the midgut, but not in the integument, foregut and hindgut of L. migratoria manilensis.

Key words: Locusta migratoria manilensis, chitinase gene, midgut, semi-quantitative RT-PCR

ZHANG Ba-Sheng, CAO Yue-Qing, ZHANG Wei, YIN You-Ping, WANG Zhong-Kang, PENG Guo-Xiong, XIA Yu-Xian. Cloning, sequence analysis and tissue expression of chitinase gene from the midgut of Locusta migratoria manilensis (Meyen)[J]., 2007, 50(6): 555-559.

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Cloning, sequence analysis and tissue expression of chitinase gene from the midgut of Locusta migratoria manilensis (Meyen)

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