Abstract: The complete mitochondrial genome of the Oriental fruit fly, Bactrocera dorsalis is important for general molecular and evolutionary studies in Tephritidae. Via sequencing and cloning the target DNA sequences, a complete mitochondrial genome sequence of B. dorsalis from the Chinese population was determined and analyzed. The results indicated that the complete mitochondrial genome of B. dorsalis is a circular molecule of 15 915 nucleotides (GenBank accession no.: DQ845759). Its overall composition is 39.3%A, 16.2%C, 10.2%G, and 34.3%T, and the gene content includes 22 tRNAs, 2 rRNAs and a long non-coding region (A+T-rich region). Seven protein genes and 13 tRNA genes are transcribed from J strand, and the other 6 protein genes and 9 tRNA genes are transcribed genes from N strand. Protein coding genes on J strand show a fairly similar A% and T%, whereas those encoded on N strand have a higher proportion of A than T. The similarity analysis of mtDNA COⅠ genes between B. dorsalis and the other 14 fruit flies showed that there was a high similarity among B. dorsalis and the other species belonging to the same subgenus Bactrocera.

Key words: Diptera, Tephritidae, Bactrocera dorsalis, mitochondrial genome