昆虫细胞系的培养和建立技术

›› 2007, Vol. 50 ›› Issue (8): 834-839.

• RESEARCH PAPERS • Previous Articles Next Articles

Advances in establishment of insect cell lines

ZHANG Huan, ZHANG Yong-An, QIN Qi-Lian, WANG Yu-Zhu, QU Liang-Jian, LI Xuan, MIAO Lin, YIN Zhen-Xian, ZHANG Ai-Jun, WEN Fa-Yuan

(State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China)

Online:2007-09-10 Published:2007-09-10
Contact: QIN Qi-Lian

Abstract

Abstract: More than 800 insect cell lines have been reported by now. Insect cell lines have become useful tools with a variety of applications in such research fields as insect pathology, parasitology, endocrinology, genetics, molecular biology, etc. This paper reviews the progresses in the technique of establishing insect cell lines in combination with our results and experiences gained in recent years, which include development of insect culture, establishment of insect cell lines, initiation of a cell line from different tissues, characterization and identification of new insect cell line.

Key words: Insect, cell culture, cell line, establishment

ZHANG Huan, ZHANG Yong-An, QIN Qi-Lian, WANG Yu-Zhu, QU Liang-Jian, LI Xuan, MIAO Lin, YIN Zhen-Xian, ZHANG Ai-Jun, WEN Fa-Yuan. Advances in establishment of insect cell lines[J]., 2007, 50(8): 834-839.

[1]	ZHANG Hai-Jian, SONG Jian, MA Hong-Xia, SHI Jie, HU Qing-Yu, WANG Zhen-Ying. Identification of Delia platura (Diptera: Anthomyiidae) and its damage to corn seeds and seedlings in Xingtang, Hebei, northern China [J]. , 2018, 61(9): 1114-1120.
[2]	LU Xiao-Peng, WU Qiong, LI Mao-Long, LI San-Xing, MA Zhi-Qing, ZHANG Xing. Screening of insecticidal activities of 126 plant essential oils or main components of essential oils against Anaphothrips obscurus (Thysanoptera: Thripidae) [J]. , 2018, 61(8): 941-949.
[3]	YUAN Rui, LI Li-Li, LI Chao, XIA Xiao-Ju, ZHENG Li, YU Yi, MEN Xing-Yuan. Toxicity and hazard assessment of six neonicotinoid insecticides on Osmia excavata (Hymenoptera: Megachilidae) [J]. , 2018, 61(8): 950-956.
[4]	LIU Rui-Ying, XIAO Zi-Jin, HE Jing-Lan, WAN Gui-Jun, PAN Wei-Dong, CHEN Fa-Jun. Population fitness of the migratory brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae) in response to the change of geomagnetic field intensity [J]. , 2018, 61(8): 957-967.
[5]	LI Xin-Mei, QIU Wu-Jie, CUI Bin, YANG Zong-Lin, SHEN Ya-Wen, LU Yi-Ping, HAN Yun-Chao, LI Dan-Dan. Cellular location of antisense oligonucleotide of snoRNA Bm-15 and its interference efficiency with Bm-15 in Spodoptera frugiperda Sf9 cells [J]. , 2018, 61(7): 795-800.
[6]	ZHANG Min, LIU Jia, FU Wei-Jie, DAI Guang-Hui. Isolation and identification of insecticidal compounds from Curcuma longa roots against Aphis craccivora (Hemiptera: Aphididae) adults [J]. , 2018, 61(6): 698-703.
[7]	CHEN Qiu-Yan, CHANG Ya-Jun, GUO Qian-Qian, SU Ran-Ran, WANG Bo, HE Jing, XIE Gui-Ying, ZHAO Xin-Cheng. Identification of insect brain neuropils aided with the application of 3D printer technology [J]. , 2018, 61(4): 439-448.
[8]	Fahimeh HAMIDI, Ali MEHRVAR, Naser EIVAZIAN KARY, Hassan VALIZADEH. Biological activities of Thymus vulgaris, Petroselinum sativum and Stachys lavandulifolia extracts against adult Callosobruchus maculatus (Coleoptera: Bruchidae) (In English) [J]. , 2018, 61(3): 331-339.
[9]	TONG Xiao-Ling, SHI Jin, GAI Ting-Ting, FANG Chun-Yan, DAI Fang-Yin. Research and application of insect promoters [J]. , 2018, 61(3): 360-370.
[10]	ZHENG Ya-Nan, LIU Pei-Xuan, SHI Yong, FAN Li-Chun. Advances in the research of arginine kinase in insects [J]. , 2018, 61(3): 385-390.
[11]	ZHAO Jing-Ru, YUAN Shou-Li, GAO Bin, ZHU Shun-Yi. Antimicrobial and insecticidal activities and evolution of inhibitor cystine knot peptides [J]. , 2018, 61(10): 1212-1221.
[12]	JIA Zhi-Rong, LIU Zhuan-Zhuan, WANG Xiao-Ming, Tricia WILLIAMS, LIU Pei-Wen, LI Xiao-Cong, YAN Gui-Yun, CHEN Xiao-Guang. Screening of the deltamethrin-resistant strain of Aedes albopictus (Diptera: Culicidae) and its susceptibility to dengue virus [J]. , 2018, 61(1): 11-17.
[13]	GAO Jing-Peng, CHEN Han-Ming, MA Ya-Jun. Establishment of diagnostic doses of three pyrethroid insecticides for detecting resistance in Aedes albopictus (Diptera: Culicidae) in China [J]. , 2018, 61(1): 18-24.
[14]	LUO Lan, CUI Feng. Establishment and identification of a new cell line from Culex pipiens quinquefasciatus (Diptera: Culicidae) [J]. , 2018, 61(1): 79-85.
[15]	ZOU Zhen, KANG Le. Vector biology and vector insects [J]. , 2018, 61(1): 1-10.

Advances in establishment of insect cell lines

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Metrics

Comments

Recommended 0