›› 2008, Vol. 51 ›› Issue (10): 1003-1010.

• RESEARCH PAPERS •

### Effects of parasitization by Nasonia vitripennis (Hymenoptera: Pteromalidae) on the compositions and contents of soluble proteins and arylphorin in host Boettcherisca peregrina (Diptera: Sarcophagidae) pupae

HAN Cheng-Xiang

• Online:2010-07-29 Published:2008-10-20

Abstract: For further exploring the physiological effects of parastization by pupa-specific parasitoids on protein metabolic efficiency of hosts, the changes in compositions and contents of soluble proteins and arylphorin in the fat body and hemolymph of Boettcherisca peregrina pupae parasitized by Nasonia vitripennis were investigated by using the  Bradford method for protein quantitation, Western blotting, and enzyme_linked immunosorbent assay. The results showed that the compositions of soluble proteins in the fat body and hemolymph of parasitized pupae were similar to those of non-parasitized pupae. There was a storage protein, namely arylphorin with a subunit molecular weight of 80 kDa, in the fat body and hemolymph of both parasitized and non-parasitized pupae. The arylphorin subunit was not degraded in the fat body of both parasitized and non-parasitized pupae, but was found to be broken up into

two bands with similar molecular weight only in the hemolymph of parasitized pupae at 12 h after parasitization by the Western blotting. This suggests that the arylphorin in the hemolymph might be broken down earlier in parasitized pupae than in non-parasitized pupae. The contents of soluble proteins in the fat body were significantly lower in parasitized pupae than in non-parasitized pupae at all the sampling stages except at 24 h after parasitization. In contrast, the contents of arylphorin in the fat body of parasitized pupae were also significantly lower than those of non-parasitized ones except at 48 h after parasitization, only 32.0% of that for nonparasitized pupae at 12 h after parasitization. The contents of soluble proteins in the hemolymph were more or less lower in parasitized pupae than in non-parasitized pupae, and their differences were significant at 2, 12 and 24 h after parasitization. There was a tendency that the content of arylphorin in the hemolymph was lower in parasitized pupae than in non-parasitized pupae, especially at 12 h after parasitization which was only 17% as high as that in non-parasitized pupae. In general, the results suggest that the parasitization by N. vitripennis may result in marked decreases in the contents of both soluble proteins and arylphorin in the fat body and hemolymph of hosts.