›› 2008, Vol. 51 ›› Issue (11): 1210-1215.
• RESEARCH PAPERS • Previous Articles Next Articles
TANG Ting
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Abstract: β-actin is a member of the actin family, which plays important roles in maintaining cytoskeletal structure, cytoplasmic movement and cell division. β-actin is one of the most commonly used internal controls in gene quantitative analysis. In this study, a full-length cDNA of β-actin from Mantichorula semenowi was cloned via homology cloning and RACE. Sequence analysis and phylogenetic study showed that this cDNA contained 1 372 nucleotides, with a 1 131 bp open reading frame (ORF) encoding 376 amino acids and flanked with a 66 bp 5′UTR and a 175 bp 3′UTR. β-actin shared a high sequence identity of 96%-99% with that of other insects. Profiles ofβ-actin expression in different recovery time after heat shock were expressed similarly and not significantly different from that of the unstimulated control, suggesting an ubiguitous expression pattern.
Key words: Mantichorula semenowi, β-actin gene, homology cloning, rapid amplification of cDNA ends(RACE), internal control
TANG Ting. Cloning, characterization and expression detection of the β-actin cDNA from Mantichorula semenowi (Coleoptera: Tenebrionidae)[J]., 2008, 51(11): 1210-1215.
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http://www.insect.org.cn/EN/Y2008/V51/I11/1210
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