›› 2008, Vol. 51 ›› Issue (12): 1293-1303.

• RESEARCH PAPERS • Previous Articles     Next Articles

Real-time fluorescent quantitative PCR technique and its applications in entomological studies

WANG Guang-Hua   

  • Online:2010-07-29 Published:2008-12-20

Abstract: Real-time fluorescent quantitative PCR is a laboratory technique for quantitative measurement of the DNA template concentration by monitoring the fluorescent signal in each of DNA amplification cycle. This technique is accurate, speedy, sensitive and specific. It has been widely used in such fields as bioengineering, quarantine, microbe identification and classification, food safety inspection and medical science. In this article, the principles, characteristics and recent advances in fluorescent probes of the real-time fluorescent quantitative PCR assay, and its applications in entomological studies were reviewed.

Key words: DNA, real-time fluorescent quantitative PCR (FQ-PCR), fluorescence resonance energy transfer (FRET), probe, entomology