›› 2008, Vol. 51 ›› Issue (3): 246-257.

• RESEARCH PAPERS • Previous Articles     Next Articles

A dense linkage map of the silkworm ( Bombyx mori )based on AFLP markers

ZHANG Lie   

  • Online:2010-07-27 Published:2008-03-20

Abstract: For studying QTL of silkworm quantitative traits, the backcross hybrid was obtained with female of a commercial race C100 crossing with male of the first filial hybrid of C100 and Dazao. Based on improved AFLP method,1 744 AFLP markers in agreement with 11 segregation ratio (P0.05) were obtained employing 96 pairs primers, and a dense linkage map composing of 36 linkage groups of 814 loci was constructed employing the Map Manager QTXb19 Version 0.29 software. The total length of the groups was 13 005 cM and the distances between loci were 2.3 to 47.7 cM. The average length of the groups was 361.25 cM and the average length between loci was 15.98 cM, and only 39 gaps whose distance between loci was more than 30 cM were in the constructed map. The number of loci in the constructed map ranged from 8 to 92 and the average loci of the groups was 23. Two groups of the constructed map correspond with the 15th and W chromosome of traditional linkage map.

Key words: Bombyx mori, molecular marker, AFLP, linkage map