›› 2008, Vol. 51 ›› Issue (8): 849-854.

• RESEARCH PAPERS • Previous Articles     Next Articles

Evolutionary analysis of CYP6 genes of Philotrypesis spp. (Hymenoptera: Chalcidoidea: Pteromalidae)

LIU Ying   

  • Online:2010-07-27 Published:2008-08-20

Abstract: Nine new DNA fragments encoding cytochrome P450 were amplified from the fig wasps Philotrypesis spp.:  CYP6CK1, CYP6AQ2, CYP6AQ3, CYP6AS20, CYP6AS21, CYP6AS22, CYP6AS23, CYP6AS24 and CYP6AS25. These sequences include helix and heme-binding regions which are important functional regions for all P450 genes. We employed MEGA 4.0 to construct gene tree and PAML 4.0 to test the selection pressure on the encoding region. The results showed that the expansion of CYP6AS, CYP6AQ and CYP6CK subfamilies might be produced by the events of gene duplication. Members in the CYP6AS subfamily from those fig wasps are suffering from purifying selection, and the encoding regions are highly conservative.

Key words: Philotrypesis, cytochrome P450, CYP6 family, gene duplication, purifying selection