›› 2009, Vol. 52 ›› Issue (1): 106-109.doi:
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Abstract: 【Aims】 To localize the specific allergens in the body of Blomia tropicalis by immunohistochemistry. 【Methods】 Paraffin sections of B. tropicalis mites stained by hatmatoxylin-osin (HE) were observed by optical microscope. Serum specific IgE antibodies from patients who were sensitive to the house dust mite served as probe, and the specific allergens in the body of B. tropicalis were localized. 【Results and Conclusion】 Immunohistochemistry staining revealed that the specific allergens were located in the gut wall, gut contents and the reproductive system, but strongly bound to the midgut wall and contents.
Key words: Blomia tropicalis, allergens, antigen localization, immunohistochemistry, hatmatoxylin-eosin stain
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http://www.insect.org.cn/EN/Y2009/V52/I1/106
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