The ultrastructure of olfactory sensilla on the antenna of Proagopertha lucidula (Faldermann) adult was studied using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The results showed that the olfactory sensilla concentrated on antennal lamellae and all these sensilla were situated in cuticle or in the sunken cavity formed by antennal cuticle depressions. Five types of olfactory sensilla in lamellae were found. There are mainly sensilla placodea (S. pl) in lamellae of P. lucidula. In addition to S. pl, there are sensilla basiconca (S. ba), sensilla cavitica (S. ca), sensilla coeloconica (S. co) and sensilla trichodea (S. tr). Based on the differences of plate and cone shape, S. pl are further classified into four subtypes, S. ba into two subtypes, and S. co into two subtypes. There are a plenty of pores and pore tubules in the cuticle of olfactory sensillum. The number of neurons in olfactory sensilla is inconsistent, including one olfactory receptor neuron (ORN), or two ORNs. The total number of sensilla in the male is 1.8 times that in the female. The number of S. bal in male is two times that in female, the number of S. co in female is four times that in male, while the number of S. pl in male is two times that in female. There is no significant difference in the number of S. co between male and female.

【Aims】 To localize the specific allergens in the body of Blomia tropicalis by immunohistochemistry. 【Methods】 Paraffin sections of B. tropicalis mites stained by hatmatoxylin-osin (HE) were observed by optical microscope. Serum specific IgE antibodies from patients who were sensitive to the house dust mite served as probe, and the specific allergens in the body of B. tropicalis were localized. 【Results and Conclusion】 Immunohistochemistry staining revealed that the specific allergens were located in the gut wall, gut contents and the reproductive system, but strongly bound to the midgut wall and contents.

To assess the insecticidal activity of eucalyptus oil and α-pinene against dried fish pest Dermestes maculates. Mortalities of its larvae and adults under different doses or treatment time were investigated by bioassay method. The results showed that the tested oil and its active ingredient (α-pinene) affected significantly (P<0.001) on the mortality of both the larvae and adults of D. maculatus. Responses varied with compound, dose and exposure time. Over 90% mortality at 72 h after treatment was achieved with the eucalyptus oil against larvae at a dose of 32 μL/cm³. Eucalyptus oil at 32 μL/cm³, were highly toxic to adults of D. maculatus 72 h after treatment. α-Pinene also showed highly toxic effects on both larvae and adults of the beetles at the same exposure time and doses. It is also interesting to mention that the adults of D. maculatus were found to be more tolerant than larvae. In probit test eucalyptus oil was much more effective than α-pinene. These naturally occurring materials could be useful for managing populations of D. maculatus.

To understand the worker policing mechanism of honeybees, we compared the surface ultrastructure of queen-laid diploid and haploid eggs of Apis cerana cerana to that of worker-laid eggs using SEM and conducted a detailed comparative analysis on chemical components of newly laid (0 h) and 5 h eggs of various types by GC-MS. The results show that the egg surface is covered with an outer layer typified by hexagonal structures filled with small nodules and there are no differences between different types of eggs. The chemical components coating queen eggs, diploid and haploid, are more abundant than those of workers. C27∶2 and C27∶1 may be the chemical pheromones on the egg surface.