›› 2010, Vol. 53 ›› Issue (5): 525-530.doi:

• RESEARCH PAPERS • Previous Articles     Next Articles

Isolation, structure identification and insecticidal activity of compounds from Ficus tsiangii Merr. ex Corner

WANG Xue-Gui, SHEN Li-Tao, TIAN Yong-Qing, XU Han-Hong   

  • Online:2010-05-20 Published:2010-05-20

Abstract:  In this study the chemical constituents of Ficus tsiangii Merr. ex Corner stem and root-bark were isolated and puified with activity-guided fractionation technology by silica gel, thin-layer chromatography and Sephadex LH-20 column chromatography to investigate the constituents with insecticidal activity, and their structures were identified with spectroscopic means. The results indicated that four bioactive compounds were isolated and their structures were identified as β-amyrin acetate, taraxerone, chrysoeriol and oleanolic acid, respectively. The insecticidal activities of these compounds(500 μg/g sugar) were assayed against Musca domestica vicina adults with stomach-toxicity test, of which the corrected mortality achieved by taraxerone was 83.33% at 48 h after treatment, showing higher insecticidal activity. The toxicity of taraxerone against M. domestica vicina adults was also evaluated with LC50 value of 89.82 μg/g sugar at 48 h after treatment, which was only a little weaker than that of the control with rotenone (LC50 value of 67.58 μg/g sugar). Therefore taraxerone can be further investigated as a biopesticide.

Key words: Ficus tsiangii, activity-guided fractionation, Musca domestica, insecticidal activity, taraxerone, biopesticide