Abstract:

With a close-range live specimen sampling technique based on solid-phase microextraction (SPME), the pheromone components of Dendrolimus houi virgin female moths during the calling period were collected in order to increase the quantities of pheromone components. The sampleloaded SPME fiber was introduced onto the gas chromatography (GC) coupled with mass spectrometry (MS) or electroantennographic detection (EAD), and the samples were analyzed under oven temperature program condition. The results indicated that large amounts of (5E, 7Z)-5, 7-dodecadien-1-ol (E5, Z7-12:OH) and (5E, 7Z)-5, 7-dodecadien-1-yl acetate (E5, Z7-12:OAc) were obtained by SPME CAR/DVB fiber, and trace amounts of (5E, 7Z)-5, 7-dodecadienal (E5, Z7-12:Ald) were also observed in the effluvia. The three compounds sampled by SPME fiber could evoke electroantennographic responses from male moth antennae. Compared with the pheromone gland extraction method with solvent, SPME sampling technique coupled with GC-MS and GC-EAD analysis has some advantages, such as representative sample collecting, relative large quantities of sample obtained, and solvent-free sample preparation technique, etc. Finally, the importance and practical aspects concerning the SPME are also discussed.

Key words: Dendrolimus houi, sex pheromone, SPME, GC-MS, GC-EAD