›› 2011, Vol. 54 ›› Issue (2): 149-156.doi:

• RESEARCH PAPERS • Previous Articles     Next Articles

Analysis of genetic diversity of different populations of Bactrocera dorsalis (Diptera: Tephritidae) using microsatellite markers

WU Zhong-Zheng, LI Hong-Mei, BIN Shu-Ying, SHEN Jian-Mei, HE Hua-Liang, LUO Mei, Ma Jun, LIN Jin-Tian   

  • Online:2011-02-20 Published:2011-03-10

Abstract: To explore the genetic variation, the source of invasion and the dispersal of different geographical populations of Bactrocera dorsalis (Hendel), the genetic diversity and relationships of 30 B. dorsalis populations consisting of 180 individuals collected from 10 provinces of South China, Thailand, Hawaii, Philippines, and Laos were analyzed with 13 microsatellite loci. The analysis results with the softwares of Popgene32 and NTSYS-pc2.10e showed that the Nei’s genetic identity of 30 B. dorsalis populations was between 0.3599 and 0.9153. A relatively high level of genetic diversity was revealed: H=0.6464±0.1026, I=1.2845±0.2632. UPGMA cluster analysis indicated that the populations from Fujian and Hainan gathered separately into two different clades, the populations from Guangdong and Taiwan gathered into one clade, while the populations from Guangxi, Thailand, Hunan, Yunnan, Laos, Sichuan, Chongqing and Guizhou formed the other one. So it is inferred that the populations from Thailand and Laos were the earliest ones to invade China, Yunnan was the region to be invaded earliest, and Guangxi was probably also among the regions to be invaded early by this pest.

Key words: Bactrocera dorsalis, geographical population, genetic diversity, genetic distance, microsatellite, UPGMA cluster analysis