Abstract: 【Aim】 Wolbachia are a group of maternally inherited intracellular bacteria that infect a wide range of arthropods and cause an array of effects on host reproduction. Recently, researchers have carried out transfer experiments that generated novel infection types to elucidate the interaction between Wolbachia and the hosts. 【Methods】 Under laboratory conditions, Wolbachia infected by the small brown planthopper, Laodelphax striatellus (Fallén), were extracted and purified, and then were transferred into the new host Tetranychus phaselus Ehara using microinjection. The effects of Wolbachia extracted from L. striatellus and the native Wolbachia on reproductive fitness and adult longevity of T. phaselus were studied, and the densities of Wolbachia during host aging were measured. 【Results】 Our results showed that the novel Wolbachia was present at a rather low density in T. phaselus, being only 0.5% of the densities of the native Wolbachia. Unlike native Wolbachia, novel Wolbachia could not induce cytoplasmic incompatibility in the T. phaselus population, but they could decrease the fecundity of T. phaselus significantly. 【Conclusion】 This study indicates that Wolbachia from L. striatellus have potential ability to inhibit population expansion of T. phaselus and will be valuable for biocontrol of T. phaselus.

Key words: Laodelphax striatellus, Tetranychus phaselus, symbiont, Wolbachia, microinjection, artificial transinfection, reproduction fitness, longevity