›› 2014, Vol. 57 ›› Issue (1): 67-73.

• RESEARCH PAPERS • Previous Articles     Next Articles

Induction of salicylic acid and jasmonic acid in cucumber leaves after infestation by Liriomyza huidobrensis (Diptera: Agromizidae) larvae

SUN Xing-Hua, ZHOU Xiao-Rong, PANG Bao-Ping*, MENG Qing-Jiu   

  1.  (College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China)
  • Online:2014-01-20 Published:2014-01-20

Abstract: 【Aim】 In order to investigate the mechanism of interactions between Liriomyza huidobrensis  (Blanchard) and its host plants and build an indispensable foundation for controlling the fly by use of inducible defenses. 【Methods】 High-performance liquid chromatography (HPLC) and ultraperformance liquid chromatography-mass spectrometry (UPLC-MS) were used to determine the effects of infestation of larval L. huidobrensis on induction of jasmonic acid (JA) and salicylic acid (SA), respectively, in cucumber leaves. 【Results】 The results showed that JA contents in the damaged leaves were significantly higher than those in intact controls only 1 d after the larval continuous infestation, and the increase magnitudes in the light-damage treatment and the severedamage treatment reached the maximum at 3-5 d after larval infestation, increasing 2.01 and 1.62 times as compared with the intact control, respectively. SA contents were significantly higher at 3 d after larval infestation, and the increase magnitudes in the lightdamage treatment and the severe-damage treatment reached the maximum at 9 d after larval infestation, rising 4.66 and 1.67 times as compared with the intact control, respectively. The induction of JA and SA was significantly systemic in the severe-damage treatment but not in the lightdamage treatment. 【Conclusion】 The results suggest that the larval infestation of L. huidobrensis can induce the JA and SA increase in cucumber leaves.

Key words: Liriomyza huidobrensis, larval infestation, cucumber, inducible defense, jasmonic acid (JA), salicylic acid (SA)