Abstract: 【Aim】This study aims to illustrate the effects of using frozen-preserved housefly (Musca domestica) pupae as hosts on the reproduction of Spalangia endius.【Methods】The survival number, offspring number and sexes of S. endius on fresh housefly pupae and the housefly pupae frozen killed and preserved at -20℃, respectively, were recorded daily, and the life tables were built. The effects of host types on the parasitism rate, offspring number and sex ratio, and parameters of life table were compared and analyzed. 【Results】The results showed that the parasitism rate, offspring number, and male proportion of S.endius on the frozen-killed housefly pupae were lower than those of wasps on the fresh housefly pupae (P<0.01). The longevity and oviposition duration of adults were similar between the two groups (P>0.05). The parasitism rate and offspring number in both groups decreased with the increasing of female age (P<0.01). The net reproductive rate (R₀) of S. endius on the fresh housefly pupae and frozen-killed pupae was 34.91 and 20.16, and their intrinsic natural increase (r_m) was 0.17 and 0.11, respectively. R₀ and r_m were both larger on the fresh housefly pupae than on frozen-killed housefly pupae (P<0.01). The mean generation time (T) and doubling time (DT) of S. endius on the frozen-killed housefly pupae were prolonged compared with those of wasps on the fresh pupae (P<0.01). 【Conclusion】Our results indicate that S. endius can use frozen-killed housefly pupae as hosts to finish life history. We believe that the method of frozen-killed hosts would be useful for massively culturing of S. endius.

Key words: Spalangia endius, Musca domestica, life table, parasitoid wasp, host preservation, life history, frozen-killed housefly pupae, fresh housefly pupae