Abstract: 【Aim】 This study aims to deeply understand the sex pheromone communication system of Holcocerus vicarius (Walker). 【Methods】 The behavioral responses of male adults of  H vicarius  to synthetic sex pheromone components and their mixtures in different ratios and at different dosages were observed in wind tunne. 【Results】 The results of single component tests showed that the lures containing only (Z) -7-tetradecenyl acetate (Z7-14:Ac) elicited all steps of behavioral responses of male adults, i.e., exciting, landing on the lure and exhibiting their hairpencils, while other four single components (E3-14:Ac, Z3E5-14:Ac, E3-14:OH and Z3-14:OH) elicited the male adults exciting only, but not flying to the sex pheromone source. The binary mixture of (E)-3-tetradecenyl acetate (E3-14:Ac) and Z7-14:Ac caused an increase in the intact sexual response proportion of males to synthetic sex pheromone components. When the lures were baited with the ternary mixture including Z7-14:Ac, E3-14:Ac and (Z,E)-3,5tetradecenyl acetate (Z3E5-14:Ac) in a 10:4:4 ratio at the dosage of 1 300 μg or 1 000 μg, they elicited 71.7% or 70.3% of males to behave hairpencils, respectively, but no significant difference between them (P＞0.05). 【Conclusion】 Wind tunnel behavioral tests of H. vicarius provide the basis for studying the sex pheromone related biology of H. vicarius and developing comprehensive prevention and control strategies by applying sex pheromone.

Key words: Holcocerus vicarius , sex pheromone, wind tunnel, behavioral responses, lure