›› 2015, Vol. 58 ›› Issue (12): 1362-1367.

• SHORT COMMUNICATIONS • Previous Articles     Next Articles

Culture of the primary cells of Apis cerana cerana larvae and the replication of Chinese sacbrood virus in the cultured cells

LI Hui, FEI Dong-Liang, HU Ying, MA Ming-Xiao*   

  1. (College of Animal Science and Veterinary Medicine, Liaoning Medical University, Jinzhou, Liaoning 121001, China)
  • Online:2015-12-20 Published:2015-12-20

Abstract: 【Aim】 To establish an applicable culture method for honeybee sourced cells and to lay the foundation for culture of honeybee cells and related virus research. 【Methods】 The premium culture medium and the corresponding fetal bovine serum (FBS) concentration were screened by comparing the growth status of the primary cells of the Chinese bee larvae cultured in Grace’s medium and WH2 medium, respectively. Then, the cultured primary cells were inoculated with Chinese acbrood virus (CSBV), and the virus replication was detected with real-time quantitative RT-PCR. 【Results】 Cells cultured in Grace’s medium were large and round, transparent, and regularly edged with no particulate matters, and had significant higher viability than those cultured in WH2 medium. The economical and applicable FBS concentration was determined to be 15% based on the comparison of the cell viability cultured in Grace’s medium with various concentrations of FBS. The inoculated CSBV virus could replicate and proliferate rapidly along with the division of the host cells. 【Conclusion】 The primary cells of Chinese bee larvae grow well in Grace’s medium with 15% FBS, in which the replication of CSBV can proceed.

Key words: Apis cerana cerana, Chinese bee Sacbrood virus (CSBV), primary cell culture, fetal bovine serum (FBS), real-time RT-PCR