Acta Entomologica Sinica ›› 2016, Vol. 59 ›› Issue (8): 839-845.doi: 10.16380/j.kcxb.2016.08.005

• RESEARCH PAPERS • Previous Articles     Next Articles

Insecticidal activity and identification of the active ingredients of Clausena lansium seed extracts against larval Spodoptera litura (Lepidoptera: Noctuidae)

GUO Cheng-Lin 1,2, QIN Liu-Yan3, WAN Shu-Qing1,*, MA Yue-Feng2, QIN Jian-Lin2, MA Yong-Lin2   

  1. (1. Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou 510642, China; 2. Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Institute of Plant Protection, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 3. Institute of Biotechnology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China)
  • Online:2016-08-20 Published:2016-08-20

Abstract: 【Aim】 By measuring the antifeedant activities of extracts from Clausena lansium seeds against Spodoptera litura larvae as well as separating and identifying the active ingredients and measuring the antifeedant activity, development deterrent activity and cytotoxicity, this study aims to provide references for screening botanical pesticides for ecological management of S. litura. 【Methods】 Leaf disc method was used to determine the no-choice antifeedant activities of methanol extract of C. lansium seeds and extracts of other four solvents (petroleum ether, ethyl acetate, n-butanol and water) as well as the developmental inhibitory activity against the 3rd instar larvae of S. litura. Silica column chromatography was used to separate the active antifeedant ingredients of petroleum ether and ethyl acetate extracts. Nuclear magnetic resonance spectroscopy (NMR) was used to identify the structure of active antifeedant compound, and MTT method was used to determine the cytotoxicity of antifeedant compound to S. litura cell lines. 【Results】 The methanol extract of C. lansium seeds exhibited strong antifeedant activity against the 3rd instar larvae of S. litura. The active ingredients were mainly distributed in petroleum ether extracts and ethyl acetate extracts. Extracts of petroleum ether and ethyl acetate were fractioned with silica column chromatography and 10 fractions were collected then. Fraction 2, a yellow crystal, showed the highest antifeedant activity. Through isolation, purification and 1HNMR and 13CNMR analyses, lansiumamide B was identified to be the main active ingredient, with the 48 h median antifeedant concentration (AFC50) of 214.95 μg/mL against the 3rd instar larvae of S. litura. This ingredient also displayed high cytotoxicity to the 3rd instar larvae of S. litura, with the 48 h cell medium inhibition concentration value of 10.09 μg/mL. 【Conclusion】 Extracts from C. lansium seeds have strong antifeedant activities and cytotoxicity against S. litura larvae. Lansiumamide B is one of the main active ingredients of the extracts, suggesting its good application prospect as a botanical insecticide.    

Key words: Spodoptera litura; Clausena lansium, extract, lansiumamide B, antifeedant activity, cytotoxicity