Acta Entomologica Sinica ›› 2017, Vol. 60 ›› Issue (1): 18-23.doi: 10.16380/j.kcxb.2017.01.003
• RESEARCH PAPERS •
YI Hua-Shan1，2， MA Xian-Ping1， PAN Min-Hui2，*， LU Cheng2，*
【Aim】 Bombyx mori inhibitor of apoptosis protein (BmIAP) has been found in the silkworm， B.mori， and characterized as a protein of the IAP (inhibitor of apoptosis protein) family. This study aims to verify its function as a suppressor of apoptosis in B.mori cells， so as to further study the roles of BmIAP in cell apoptosis. 【Methods】 The transient expression vector named PIZ/V5-BmIAP-dsRed was transfected in the BmN-SWU1 cells of B.mori by liposome， and the mRNA and protein expression levels of BmIAP gene were evaluated by real-time quantitative PCR and Western blot analysis， respectively. The BmN-SWU1 cells were induced by 150 ng/mL actinomycin D for 12， 18 and 24 h， and the relationship between BmIAP protein and cell apoptosis was assayed by immunofluorescence and real-time quantitative PCR methods. 【Results】 The PIZ/V5-BmIAP-dsRed vector effectively expressed BmIAP fusion protein of about 60 kD， and the mRNA expression level of BmIAP was up-regulated 45-fold in BmN-SWU1 cells of B.mori. After BmIAP was transiently expressed for 72 h using PIZ/V5-BmIAP-dsRed vector in the BmN-SWU1 cells， BmIAP significantly inhibited the apoptosis in the BmN-SWU1 cells at 12 h after induction by actinomycin D. 【Conclusion】 BmIAP can inhibit apoptosis in B.mori cells and is one of the inhibitor of apoptosis proteins in B.mori.
Bombyx mori； apoptosis； inhibitor of apoptosis protein(IAP)； eukaryotic expression,
YI Hua-Shan, Ma Xian-Ping, Pan Min-Hui, Lu Cheng. Eukaryotic expression and function analysis of Bombyx mori inhibitor of apoptosis protein (BmIAP) gene[J].Acta Entomologica Sinica, 2017, 60(1): 18-23.
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