Acta Entomologica Sinica ›› 2017, Vol. 60 ›› Issue (4): 412-420.doi: 10.16380/j.kcxb.2017.04.006

• RESEARCH PAPERS • Previous Articles     Next Articles

Southern rice black-streaked dwarf virus-induced volatiles from rice plants and behavioral responses of adult Sogatella furcifera (Hemiptera:Delphacidae) to the components of these volatiles

WANG Lu-Feng, HU Kui, HE Hua-Liang, DING Wen-Bing, LI You-Zhi*   

  1. (College of Plant Protection, Hunan Agricultural University, Changsha 410128, China)
  • Online:2017-04-20 Published:2017-04-20

Abstract: 【Aim】 Southern rice black-streaked dwarf virus (SRBSDV) is a persistent virus transmitted by the whitebacked planthopper (WBPH),Sogatella furcifera, among rice plants. This research aims to investigate whether SRBSDV can induce the changes in the types and contents of volatiles from rice plants, and the possible relationship between the host orientation behavior of WBPH and the volatile changes. 【Methods】The volatiles from SRBSDV-infected and  uninfected plants were respectively collected by using the dynamic headspace, and analyzed on GC-MS. The behavioral responses of WBPH to the volatiles detected exclusively in the infected plants were tested by using olfactometer.【Results】 Thirty-six volatile compounds belonging to 11 categories weredetected from the uninfected plants. Thirty-seven compounds belonging to 12 categories were detected from the SRBSDV-infected plants. Twelve compounds, i.e., n-hexadecane, didecyl ether, butyl octyl phthalate,n-octadecane, diisobutyl adipate (DA), pentadecanal, methyl hexadecanoate (MH), 2-methylhexacosane, benzophenone, cedrol,n-eicosane and plant alcohol, were detected exclusively in the infected plants. Meanwhile, six compounds, i.e., 1-tricosene, octadecanal,naphthalene, perhydrofarnesyl acetone, lauryl alcohol and butylated hydroxytoluene (BHT), were detected both in the infected and uninfected plants, but had significant differences in their contents. Olfactory selection experiments showed that only plant alcohol exhibited distinctly repellent activity against WBPH at the concentration of 50 μL/L.【Conclusion】 The changes in the types and contents of rice volatiles are related to rice varieties and its developmental stages. Plant alcohol is only detected in the rice variety Rongyouhuazhan at the booting stage and has significantly repellent effects on WBPH. However, more research is needed to clarify whether plant alcohol can be helpful for the transmission of SRBSDV.

Key words: Sogatella furcifera, Southern rice black-streaked dwarf virus (SRBSDV), rice volatiles, olfactory behavior, repellent effect