Acta Entomologica Sinica ›› 2021, Vol. 64 ›› Issue (9): 1080-1091.

• RESEARCH PAPERS •

### Effects of knocking down neuropeptide F gene (npf) on the feeding, growth and reproduction of Ostrinia furnacalis (Lepidoptera: Pyralididae)

SHI Jian#, WANG Yuan#, LIANG Jia, DU Juan, ZHAO Zhang-Wu*

1.  (Department of Entomology and MOA Key Laboratory of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China)
• Online:2021-09-20 Published:2021-09-03

Abstract: 【Aim】 The neuropeptide F (NPF), a type of neuropeptides in invertebrates named based on its C-terminal phenylalanine (F), participates in the regulation of many physiological functions in insects such as feeding, biological rhythm, learning and memory. The objective of this study is to clarify the effects of NPF on the growth and development of the Asian corn borer, Ostrinia furnacalis, so as to provide an important basis for pest control. 【Methods】 A method based on the efficient synthesis of dsRNA targeting genes by engineering bacteria was used to efficiently and economically knock down npf. Low concentration (0.01%) and high concentration (0.02%) of dsNPF and dsGFP (the control) were separately fed to the early 1st, 3rd and 5th instar larvae of O. furnacalis until pupation. The average feeding amount, body weight, body length, survival rate and pupation rate of the 5th instar larvae, as well as the emergence rate of pupae and the number of eggs laid by adults were detected. Besides, the developmental duration at different larval instars and pupal stage and adult life span were also detected. 【Results】 When the early 1st, 3rd and 5th instar larvae of O. furnacalis were fed with 0.01% and 0.02% dsNPF, the feeding amount, body weight, body length, survival rate and pupation rate of the 5th instar larvae, the emergence rate of pupae and the number of eggs laid per female adult significantly decreased compared with the control fed with the corresponding concentrations of dsGFP. The developmental duration of larvae and pupae in the treatment groups were significantly prolonged, and the life span of adults was significantly shorter. The earlier the larval instar was treated with dsNPF, the greater the impact on development. Among them, the 1st instar larvae treated with 0.01% dsNPF and the 3rd instar larvae treated with 0.02% dsNPF had 90% of their individuals died in the pupal stage, and the 1st instar larvae treated with 0.02% dsNPF had 90% of their individuals died in the larval stage. 【Conclusion】 The results suggest that NPF plays a regulation role in the development and feeding of O. furnacalis, providing a basis for the exploration of new green pest control.