›› 1999, Vol. 42 ›› Issue (2): 120-125.
• RESEARCH PAPERS • Previous Articles Next Articles
Li Xiao-feng,Qi Yi-peng
Online:
Published:
Abstract: Transfer vector p35IE1Neo containing anti-apoptotic p35 gene and neomycinresistant gene(as selection marker) was constructed. After transfecting Sf9 cells and G418 screening, cells transformed with p35IE1Neo were obtained. One clone was picked and named Sf9-35. By PCR detection, it was found that the genome of Sf9-35 cells contain p35 gene. After actinomycin D treatment and cellular DNA electrophoresis and detection of TUNEL(TdT mediated bio-dUTP nick endlabeling), Sf9-35 cells were found to be resistant to apoptosis induced by actinomycin D.
Key words: anti-apoptosis, insect cells, p35 gene
Li Xiao-feng,Qi Yi-peng. Stable transformation with anti-apoptotic[J]., 1999, 42(2): 120-125.
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