昆虫学报 ›› 2016, Vol. 59 ›› Issue (2): 185-191.doi: 10.16380/j.kcxb.2016.02.008

• 研究论文 • 上一篇    下一篇

中华蜜蜂嗅觉受体AcerOrco的表达及定位分析

张中印1, 杨珊珊2, 孟娇2, 赵慧婷3, 姜玉锁2,*   

  1. (1. 河南科技学院资源与环境学院, 河南新乡 453003; 2. 山西农业大学动物科技学院, 山西太谷 030801;3. 山西农业大学生命科学学院, 山西太谷 030801)
  • 出版日期:2016-02-20 发布日期:2016-02-20
  • 作者简介:张中印, 男, 1965年7月生, 河南平舆人, 副教授, 研究方向为蜜蜂饲养管理学, E-mail: zzy206@126.com

Expression and localization analysis of olfactory receptor AcerOrco in the Chinese honeybee, Apis cerana cerana (Hymenoptera: Apidae)

ZHANG Zhong-Yin1, YANG Shan-Shan2, MENG Jiao2, ZHAO Hui-Ting3, JIANG Yu-Suo2,*   

  1. (1. College of Resources and Environment, Henan Institute of Science and Technology, Xinxiang, Henan 453003, China; 2. College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China; 3. College of Life Science, Shanxi Agricultural University, Taigu, Shanxi 030801, China)
  • Online:2016-02-20 Published:2016-02-20

摘要: 【目的】通过对中华蜜蜂 Apis cerana cerana 嗅觉受体AcerOrco的表达及蛋白定位分析,阐明AcerOrco的表达特性,以期为进一步探索其功能提供理论依据。【方法】分别通过qRT-PCR技术和Western blot技术对中华蜜蜂气味受体AcerOrco mRNA及蛋白在内勤蜂和采集蜂5个组织部位(触角、头、胸、腹和足)中的相对表达量进行分析,采用免疫组化技术对AcerOrco蛋白的表达进行定位分析。【结果】定量结果显示,AcerOrco转录本在内勤蜂和采集蜂各组织中均有表达,其中触角中的表达量最高;该基因在采集蜂各组织中的表达量普遍高于内勤蜂。AcerOrco受体蛋白在内勤蜂和采集蜂各组织中也均有表达,在触角和头部的表达量明显高于其他组织。定位结果显示,在内勤蜂触角中,AcerOrco主要在毛形感器中表达,板形感器中表达不明显;在采集蜂触角的板形感器和毛形感器中均有表达,但毛形感器中的表达更多一些。【结论】获得了AcerOrco mRNA及其蛋白在内勤蜂和采集蜂各组织中的表达特性,并将这一蛋白表达定位于工蜂触角的毛形感器和板形感器中。

关键词: 中华蜜蜂, 嗅觉受体, mRNA表达, 蛋白表达, 蛋白定位

Abstract: 【Aim】 By the expression and localization analysis of olfactory receptor AcerOrco in the Chinese honeybee, Apis cerana cerana, we researched the expression characteristics of AcerOrco, in order to provide a theoretical basis for further exploring its function. 【Methods】 Real-time PCR and Western blot technology were used respectively to determine the expression profiles of AcerOrco mRNA and protein in five tissues (antennae, head, thorax, abdomen and legs)of nurse and forager bees. By immunohistochemical technique, cellular localization of the olfactory receptor in antenna was analyzed.【Results】 The expression profiling showed that AcerOrco transcripts were detected in all the tested tissues of A. cerana cerana, and the most transcripts were found in antenna. The expression of this gene in different tissues of forager was generally higher than that in nurse. AcerOrco protein were also detected in all the tested tissues, and mainly expressed in antenna and head of nurse and forager. The location result showed that in nurse AcerOrco was detected in sensilla trihodea, but had no obvious expression in sensilla placodea. In forager AcerOrco was mainly expressed in sensilla placodea and sensilla trihodea, with higher expression level in sensilla trihodea. 【Conclusion】 The expression profiles of AcerOrco at mRNA and protein levels were obtained, and the encoded protein was successfully localized in sensilla placodea and sensilla trihodea of antenna of worker bee.

Key words: Apis cerana cerana, olfactory receptor, mRNA expression, protein expression, protein localization