›› 2017, Vol. 60 ›› Issue (5): 594-603.doi: 10.16380/j.kcxb.2017.05.012

• 综述 • 上一篇    下一篇

保幼激素对昆虫变态发育调控的分子机制

何倩毓1, 张原熙2, 裴泽华3, 李美鑫1, 张旭1,*   

  1. (1. 黑龙江八一农垦大学动物科技学院, 黑龙江大庆 163319; 2. 大庆市环保局环境监测中心站, 黑龙江大庆 163316; 3. 黑龙江八一农垦大学食品学院, 黑龙江大庆163319)
  • 出版日期:2017-05-20 发布日期:2017-05-20

Molecular mechanisms of juvenile hormone in insect metamorphosis

HE Qian-Yu1, ZHANG Yuan-Xi2, PEI Ze-Hua3, LI Mei-Xin1, ZHANG Xu1,*   

  1. (1. College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China; 2. Environmental Monitoring Center Station, Daqing Environmental Protection Agency, Daqing, Heilongjiang 163316, China; 3. College of Food Science, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China)
  • Online:2017-05-20 Published:2017-05-20

摘要: 近年来随着保幼激素(juvenile hormone, JH)核受体Methoprene tolerant (Met)被鉴定,JH对昆虫变态发育调控的分子机制的研究取得了极大的进展。本文在介绍Met的鉴定以及分子伴侣Hsp83和核孔蛋白Nup358对Met亚细胞定位调控的基础上,重点阐述了JH-Met-Kr-h1-Br信号通路在完全变态昆虫幼虫至蛹变态过程中的作用以及JH-Met-Kr-h1-E93信号通路在不完全变态昆虫和完全变态昆虫成虫羽化过程中的作用。此外,Met与蜕皮激素(20-hydroxyecdysone, 20E)受体复合物EcR/USP的结合、Tai/SRC/FISC分别与Met和EcR/USP结合形成JH功能受体和20E功能受体复合物、JH对20E下游基因E75A的诱导以及USP与JH的结合等分子间的相互作用在JH与20E的互作中所产生的影响也将逐一进行论述。本文还对JH通过膜受体激活PKC和PLC等下游信号通路而发挥生理功能的研究进展进行了概述。

关键词: 保幼激素, 受体, 变态发育, 分子机制, 膜受体, 信号通路

Abstract: Since Methoprene tolerant (Met) was identified as the juvenile hormone receptor, great progress has been made in understanding the molecular mechanisms of juvenile hormone (JH) in insect metamorphosis. In this article, we summarized the identification of Met and the regulation of chaperone protein Hsp83 and Nuclearporin Nup358 on Met cellular localization, focusing on the roles of JH-Met-Kr-h1-Br signal pathway in larval-pupal metamorphosis of holometabolous insects, and JH-Met-Kr-h1-E93 signal pathway in adult metamorphosis of hemimetabolous and holometabolous insects. Moreover, the roles of many molecular interactions in JH-20E crosstalk were discussed. These interactions include the binding between Met and 20-hydroxyecdysone nuclear receptor EcR/USP, the interaction of Tai/SRC/FISC with Met and EcR/USP to form JH and 20E functional receptors respectively, the activation of JH on 20E responsive gene E75A, and the association between JH and USP. The research progress of the mechanism that JH plays its physiological function through binding to the membrane receptor to activate PKC and PLC signalling was also overviewed.  

Key words: Juvenile hormone, receptor, metamorphosis, molecular mechanism, membrane receptor, signal pathway