昆虫学报 ›› 2020, Vol. 63 ›› Issue (5): 639-648.doi: 10.16380/j.kcxb.2020.05.013

• 简 报 • 上一篇    下一篇

不同地区田间草地贪夜蛾卵巢的结构和发育

冯波1, 郭前爽2, 毛必鹏1, 钟玲3, 宋建辉3, 肖明徽3, 杜永均2,*   

  1. (1. 温州医科大学健康与环境生态研究所, 浙江温州 325035; 2. 浙江大学农药与环境毒理研究所, 杭州 310058; 3. 江西省植保植检局, 南昌 330000)
  • 出版日期:2020-05-20 发布日期:2020-06-08

Ovary structure and development of Spodoptera frugiperda (Lepidoptera: Noctuidae) from fields in different regions of China

FENG Bo1, GUO Qian-Shuang2, MAO Bi-Peng1, ZHONG Ling3, SONG Jian-Hui3, XIAO Ming-Hui3, DU Yong-Jun2,*    

  1. (1. Institute of Health and Environmental Ecology, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China; 2. Institute of Pesticide and Environmental Toxicology, Zhejiang University, Hangzhou 310058, China; 3. Jiangxi Province Station of Plant Protection and Quarantine, Nanchang 330000, China)
  • Online:2020-05-20 Published:2020-06-08

摘要: 【目的】探明田间草地贪夜蛾Spodoptera frugiperda卵巢的发育状态及其变化,为草地贪夜蛾种群的发生和迁飞动态提供精准监测依据。【方法】采用雌性生殖系统解剖法,比较不同日龄和地区的田间草地贪夜蛾雌蛾卵巢的形态差异,分析卵巢成熟状态与雌蛾交配的关系。【结果】草地贪夜蛾雌蛾生殖系统包含卵巢、输卵管、交配囊、导精管、受精囊、附腺和产卵器。根据形态特征可以将田间草地贪夜蛾卵细胞分为卵黄发生前期、卵黄期和成熟期3个时期。田间雌蛾1日龄卵巢中发育最快的卵仅处于卵黄期中期,卵巢管柄为空;3日龄卵巢出现分化,一部分卵巢仍然和1日龄相似,另一部分卵巢中存在成熟卵;这种卵巢发育分化到羽化后11 d时仍然存在。云南江城、弥勒,广西田阳以及浙江瑞安和镇海等地的田间雌蛾,到死亡时分别有61.5%, 51.7%, 41.7%, 42.1%和35.5%卵巢发育不成熟。室内饲养第1代雌蛾中,有39.6%的个体到死亡时卵巢未发育成熟,和田间雌蛾比例相似。交配雌蛾卵巢可以是发育成熟和未成熟的,但是未成熟卵巢的比例仅为18.0%±5.0%。【结论】结果提示同一代次的田间草地贪夜蛾可能同时存在迁飞和非迁飞个体,其比例随地理位置和季节发生变化。本研究的结果在一定程度上解释了单纯依据化学农药难以防治草地贪夜蛾的原因,也为田间草地贪夜蛾的迁飞监测和绿色防控提供了一定依据。

关键词: 草地贪夜蛾, 野外种群, 卵巢, 形态, 发育, 迁飞

Abstract: 【Aim】 This study aims to explore and understand the ovary developmental status of Spodoptera frugiperda and its variation in fields so as to provide accurate monitoring basis for the occurrence and migration dynamics of S. frugiperda populations. 【Methods】 The morphological differences of female ovary of S. frugiperda at different ages (day-old) and from fields of different regions in China were compared by systematic dissection of female reproductive system. The relationship between ovarian maturation and mating of female moths was analyzed. 【Results】 The reproductive system of female moth of S. frugiperda is composed of ovaries, oviducts, copulatory pouch, afferent duct, spermatheca, accessory glands and ovipositors. According to the morphological characteristics, eggs of field female moths can be divided into three stages: pre-vitellogenesis, vitellogenesis and maturation. The eggs in the ovaries of 1-day old female moths barely reach the middle stage of vitellogenesis with the ovarian stalk empty. The ovaries start to differentiate at the 3-day old female moth stage. Some ovaries in the 3-day old female moths are still similar to those in the 1-day old moths, but some ovaries have mature eggs. The differentiation of ovary lasts till the 11-day old female moth stage. Some female moths from fields of Jiangcheng County and Mile County of Yunnan Province, Tianyang County of Guangxi Province, and Rui′an County and Zhenhai County of Zhejiang Province had immature ovaries until death, which accounted for 61.5%, 51.7%, 41.7%, 42.1% and 35.5% of the total ovaries, respectively. A proportion of 39.6% of the first generation female moths reared indoors still had immature ovaries at the time of death, which was similar to that of the field female moths. The ovaries of mated female moths could be mature and immature, but the proportion of immature ovaries was only 18.0%±5.0%. 【Conclusion】 The results suggest that field female moths of S. frugiperda have a mixture of migratory and non-migrating individuals in the same generation, and their proportions vary with the location and season. The results of this study explain to some extent the uncertainty of occurrence and damage of S. frugiperda populations and provide a basis for monitoring the migratory status and green control of S. frugiperda in the field.

Key words: Spodoptera frugiperda, natural population, ovary, morphology, development, migration