关键词: 褐飞虱, 抗药性, 玻璃瓶药膜法, 快速检测, 诊断剂量

Abstract: 【Aim】 This study aims to develop a rapid detection technology for the resistance of the brown planthopper, Nilaparvata lugens, to commonly used insecticides, and to master the resistance level of its field populations in real time, so as to guide the selection and use of pesticides for control of N. lugens. 【Methods】 Based on the glass vial bioassay, a rapid detection kit for the resistance of the 3rd instar nymphs of N. lugens to five insecticides, imidacloprid, acetamiprid, ethofenprox, chlorpyrifos and isoprocarb, was developed. Correlation analysis between the mortality rate measured by the rapid detection kit and the resistance ratio measured by the rice seedling dipping method was conducted. Additionally, the accuracy of the resistance levels of the field populations of N. lugens to the five insecticides using the detection kit was verified. 【Results】 The LD₉₀ values of imidacloprid, acetamiprid, etofenprox, chlorpyrifos and isoprocarb to the 3rd instar nymphs of the laboratory susceptible strain of N. lugens at 1 h after treatment were 30.96, 92.05, 117.24, 514.21 and 1.24 ng/cm², respectively. Exposure to imidacloprid, acetamiprid, etofenprox, chlorpyrifos and isoprocarb at the diagnostic doses, the corrected mortality rates of the 3rd instar nymphs of the field populations of N. lugens in Guizhou province were 23.75%-78.75%, 25.00%-78.75%, 43.75%-88.75%, 36.25%-85.00% and 18.75%-67.50%, respectively. The correlation analysis showed that the mortality rate of the 3rd instar nymphs of the above field populations of N. lugens was negatively correlated with the resistance ratio determined by the rice seedling dipping method, and the correlation coefficient ranged from 0.8751 to 0.9754. Meanwhile, the inferred resistance ratios of N. lugens populations from Anlong (AL) region of Guizhou Province to imidacloprid, acetamiprid, etofenprox, chlorpyrifos and isoprocarb calculated by the correlation equations using the detection kit were 7.23, 3.68, 4.14, 4.12 and 31.18, respectively, and the actual resistance ratios of the above five insecticides detected by the rice seedling dip method were 6.33, 5.24, 3.71, 4.50 and 26.56, respectively, indicating that the inferred resistance ratios are consistent with the actual resistance ratios. 【Conclusion】 This rapid detection kit can quickly assess the insecticide resistance level 
of the field population of N. lugens by measuring the mortality rate of the field population.

Key words: Nilaparvata lugens, resistance, glass vial bioassay, rapid detection, diagnostic dose