取食施硅水稻对褐飞虱成虫体内保护酶和解毒酶活性的影响

doi:10.16380/j.kcxb.2022.11.008

昆虫学报 ›› 2022, Vol. 65 ›› Issue (11): 1469-1476.doi: 10.16380/j.kcxb.2022.11.008

取食施硅水稻对褐飞虱成虫体内保护酶和解毒酶活性的影响

韩永强¹, 李丹丹¹, 邓权权¹, 侯茂林^2,*

(1. 宜春学院生命科学与资源环境学院, 江西省作物生长发育调控重点实验室, 江西宜春 336000; 2. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193)

出版日期:2022-11-20 发布日期:2022-12-02

Effects of silicon amendment to rice plants on the activities of protective and detoxification enzymes in Nilaparvata lugens (Hemiptera: Delphacidae) adults feeding on the treated rice plants

HAN Yong-Qiang¹, LI Dan-Dan¹, DENG Quan-Quan¹, HOU Mao-Lin^2,*

(1. Key Laboratory of Crop Growth and Development Regulation of Jiangxi Province, College of Life Sciences and Resource Environment, Yichun University, Yichun, Jiangxi 336000, China; 2. State Key Laboratory of Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China)

Online:2022-11-20 Published:2022-12-02

摘要/Abstract

摘要： 【目的】本研究旨在明确褐飞虱Nilaparvata lugens成虫取食施硅水稻对其体内保护酶及解毒酶活性的影响，为硅介导的水稻抗虫性的应用提供证据。【方法】以TN1(感虫品种)为供试水稻品种，采用营养液添加Na₂SiO₃·9H₂O的方法设置112 mg Si/L(Si+)和0 mg Si/L(Si-)2种施硅水平，通过酶活性分析测定取食Si+或Si-水稻植株24, 48, 72和96 h后褐飞虱成虫体内保护酶［过氧化氢酶(catalase, CAT)、过氧化物酶(peroxidase, POD)和超氧化物歧化酶(superoxide, SOD)］以及解毒酶［谷胱甘肽S-转移酶(glutathione-S-transferase, GST)、羧酸酯酶(carboxylesterase, CarE)和多功能氧化酶(mixed-functional oxidase, MFO)］活性的动态变化。【结果】与取食Si-水稻植株的褐飞虱成虫相比，取食Si+水稻植株24和48 h时，褐飞虱成虫POD活性分别显著增加101.2%和55.2%，SOD活性分别显著增加78.2%和19.6%；取食72 h时，CAT和SOD活性分别显著增加16.5%和29.7%；取食96 h时，CAT和POD活性稍有降低，SOD活性显著降低12.6%。添加硅总体上增加GST和MFO活性，降低CarE活性。取食Si+水稻植株24, 48, 72和96 h时，GST活性分别显著升高57.2%, 200.7%, 84.7%和45.9%；MFO活性在取食72和96 h时分别显著升高70.2%和28.3%；而CarE活性在取食72和96 h时分别显著降低38.1%和32.0%。【结论】取食施硅水稻引起褐飞虱成虫体内保护酶和解毒酶活性发生了变化，进而对褐飞虱生理代谢产生了影响。

关键词: 褐飞虱, 硅, 水稻, 保护酶, 解毒酶

Abstract: 【Aim】 This study aims to explore the effects of silicon (Si) amendment to rice plants on the activities of protective and detoxification enzymes in the brown planthopper (Nilaparvata lugens) adults feeding on the treated rice plants, so as to provide evidence for the utilization of Si-mediated resistance in the management of insect pests in rice crops. 【Methods】 N. lugens adults feeding on susceptible rice plants (cultivar TN1) grown in nutrient solutions added with 112 mg Si/L sodium silicate (Na₂SiO₃·9H₂O)(Si+) or without addition of sodium silicate (Si-). The activities of protective enzymes ［catalase (CAT), peroxidase (POD) and superoxide (SOD)］ and detoxification enzymes ［glutathione-S-transferase (GST), carboxylesterase (CarE) and mixedfunctional oxidase (MFO)］ in N. lugens adults after feeding on the Si+ and Si- rice plants (hereafter referred as Si+ and Si- adults, respectively) for 24, 48, 72, and 96 h were measured by enzyme activity assay. 【Results】 In the Si+ adults at 24 and 48 h feeding on the rice plants the POD activities were significantly increased by 101.2% and 55.2%, respectively, and the SOD activity by 78.2% and 19.6%, respectively, as compared to those in the Si- adults. The activities of CAT and SOD in the Si+ adults at 72 h after feeding on the rice plants were significantly increased by 16.5% and 29.7%, respectively, as compared to those in the Si- adults. When the feeding duration was elongated to 96 h, the activities of CAT and POD in the Si+ adults declined slightly, and their SOD activity significantly decreased by 12.6%, as compared to those in the Si- adults. The addition of Si generally increased the activities of GST and MFO but decreased the CarE activity. In the Si+ adults, the GST activities increased significantly by 57.2%, 200.7%, 84.7% and 45.9%, respectively, at 24, 48, 72 and 96 h after feeding, the MFO activities increased significantly by 70.2% and 28.3%, respectively, at 72 and 96 h after feeding, whereas the CarE activities significantly decreased by 38.1% and 32.0%, respectively, at 72 and 96 h after feeding, as compared to those in the Si- adults. 【Conclusion】 Si amendment to rice plants influences the activities of protective and detoxification enzymes in N. lugens adults feeding on the treated rice plants, which may in return affect the physiological metabolism of N. lugens.

Key words: Nilaparvata lugens, silicon, rice, protective enzymes, detoxification enzymes

韩永强, 李丹丹, 邓权权, 侯茂林. 取食施硅水稻对褐飞虱成虫体内保护酶和解毒酶活性的影响[J]. 昆虫学报, 2022, 65(11): 1469-1476.

HAN Yong-Qiang, LI Dan-Dan, DENG Quan-Quan, HOU Mao-Lin. Effects of silicon amendment to rice plants on the activities of protective and detoxification enzymes in Nilaparvata lugens (Hemiptera: Delphacidae) adults feeding on the treated rice plants[J]. Acta Entomologica Sinica, 2022, 65(11): 1469-1476.

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取食施硅水稻对褐飞虱成虫体内保护酶和解毒酶活性的影响

Effects of silicon amendment to rice plants on the activities of protective and detoxification enzymes in Nilaparvata lugens (Hemiptera: Delphacidae) adults feeding on the treated rice plants

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