基于转录组数据的中华按蚊细胞色素P450超家族基因不同发育阶段、成蚊不同组织和吸血前后不同时期雌成蚊的表达模式分析

doi:10.16380/j.kcxb.2023.11.003

摘要/Abstract

摘要： 【目的】本研究以中华按蚊Anopheles sinensis细胞色素P450超家族基因为研究对象，分析其在中华按蚊不同发育阶段、成蚊不同组织和雌成蚊吸血前后不同时期的表达模式，为进一步研究其功能奠定基础。【方法】收集中华按蚊实验室溴氰菊酯敏感品系(WX-LS)不同发育阶段(卵、1-4龄幼虫、雄蛹、雌蛹、雄成蚊和雌成蚊)、成蚊组织(触角、唾液腺、中肠、马氏管、精巢、卵巢、表皮和脂肪体)和雌成蚊吸血后1, 3, 6, 12, 24和48 h时的样本，采用Illumina HiSeq^TM2000进行转录组测序。基于测得的转录组数据，分析细胞色素P450基因在中华按蚊不同发育阶段、成蚊不同组织及吸血前后雌成蚊中的表达量。【结果】中华按蚊细胞色素P450超家族基因的表达具有发育阶段特异性和组织特异性。在幼虫、蛹和成虫期，分别有40, 26和35个基因表达量较高，其中CYP6AG1, CYP6AG2, CYP6AH1, CYP6Z3, CYP6Y2, CYP6P4, CYP9J3和CYP9J4在整个幼虫期高表达。中华按蚊CYP6AA1, CYP6AH1, CYP6M4, CYP9J3, CYP9J4和CYP12F3在成蚊中肠和马氏管中均高表达，CYP4G16, CYP6AA1, CYP6AH1, CYP6Y1, CYP6Z3, CYP9J3和CYP9J4在成蚊所有组织中均表现出高表达。CYP4G16, CYP6AA1, CYP6AG2, CYP6AH1, CYP6Y1, CYP6Y2, CYP9K1, CYP12F3和CYP304B1在中华按蚊雌成蚊吸血后1, 3, 6, 12, 24和48 h时均高表达。【结论】在幼虫期高表达的细胞色素P450超家族基因可能参与了对幼虫期食源性有毒化合物质的解毒，在雌成蚊中肠和马氏管中高表达的细胞色素P450基因可能在解毒过程中发挥了重要作用。研究结果为进一步探究细胞色素P450超家族基因在中华按蚊生长发育、表达调控和杀虫剂代谢机制等方面的功能奠定了前期基础。

关键词: 中华按蚊, 细胞色素P450, 表达模式；发育阶段特异性, 组织特异性

Abstract: 【Aim】In this study, the cytochrome P450 superfamily genes of Anopheles sinensis were used to analyze their expression pattern in different developmental stages, different adult tissues and female adult at different periods before and after blood meal of A. sinensis, so as to lay the foundation for further studies on the function of the cytochrome P450 superfamily genes.【Methods】 Samples were collected from A. sinensis laboratory deltamethrin-susceptible strain (WX-LS) at different developmental stages (egg, 1st-4th instar larvae, male pupa, female pupa, male adult and female adult), adult tissues (antenna, salivary gland, midgut, Malpighian tubules, testis, ovary, cuticle and fat body), and female adult at 1, 3, 6, 12, 24, and 48 h after blood meal to perform transcriptome sequencing using Illumina HiSeq^TM2000. Based on the sequenced transcriptome data, the expression levels of cytochrome P450 genes in different developmental stages, different adult tissues and female adult before and after blood meal of A. sinensis were analyzed. 【Results】 The expression of the cytochrome P450 superfamily genes in A. sinensis was both developmental stage and tissue specific. In the larval, pupal and adult stages, 40, 26 and 35 genes were expressed at high levels, with CYP6AG1, CYP6AG2, CYP6AH1, CYP6Z3, CYP6Y2, CYP6P4, CYP9J3 and CYP9J4 being highly expressed throughout the larval stage. In A. sinensis adult, CYP6AA1, CYP6AH1, CYP6M4, CYP9J3, CYP9J4 and CYP12F3 were highly expressed in the midgut and Malpighian tubules, and CYP4G16, CYP6AA1, CYP6AH1, CYP6Y1, CYP6Z3, CYP9J3 and CYP9J4 were highly expressed in all adult tissues. CYP4G16, CYP6AA1, CYP6AG2, CYP6AH1, CYP6Y1, CYP6Y2, CYP9K1, CYP12F3 and CYP304B1 in female adult of A. sinensis were highly expressed at 1, 3, 6, 12, 24 and 48 h after blood meal. 【Conclusion】Cytochrome P450 superfamily genes highly expressed in the larval stage may be involved in the detoxification of food-borne toxic chemicals, and those highly expressed in the midgut and Malpighian tubules of female adult may play an important role in the detoxification process. These results lay a preliminary foundation for further investigation of the functions of cytochrome P450 superfamily genes in the growth and development, expression regulation and insecticide metabolism mechanism of A. sinensis.

Key words: Anopheles sinensis, cytochrome P450, expression pattern, developmental stage specific, tissue specific

郭迎澳, 陈斌. 基于转录组数据的中华按蚊细胞色素P450超家族基因不同发育阶段、成蚊不同组织和吸血前后不同时期雌成蚊的表达模式分析[J]. 昆虫学报, 2023, 66(11): 1443-1450.

GUO Ying-Ao, CHEN Bin. Expression profiling of the cytochrome P450 superfamily genes in different developmental stages, tissues and female adults at different periods before and after blood meal of Anopheles sinensis (Diptera: Culicidae) based on the transcriptomic data[J]. Acta Entomologica Sinica, 2023, 66(11): 1443-1450.

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基于转录组数据的中华按蚊细胞色素P450超家族基因不同发育阶段、成蚊不同组织和吸血前后不同时期雌成蚊的表达模式分析

Expression profiling of the cytochrome P450 superfamily genes in different developmental stages, tissues and female adults at different periods before and after blood meal of Anopheles sinensis (Diptera: Culicidae) based on the transcriptomic data

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