大蒜E素调控斑翅果蝇产卵选择及适合度

doi:10.16380/j.kcxb.2024.03.008

昆虫学报 ›› 2024, Vol. 67 ›› Issue (3): 374-383.doi: 10.16380/j.kcxb.2024.03.008

大蒜E素调控斑翅果蝇产卵选择及适合度

李一璇^1,#, 李隆琦^1,#, 张晟¹, 鲍悦月¹, 刘安琪¹, 庄晶晶¹, 姜标², 刘威^1,*

(1. 安徽农业大学植物保护学院, 合肥 230036; 2. 中国科学院上海有机化学研究所, 上海 200032)

出版日期:2024-03-20 发布日期:2024-04-17

Allicin E modulates oviposition preference and fitness in Drosophila suzukii (Diptera: Drosophilidae)

LI Yi-Xuan^1,#, LI Long-Qi^1,#, ZHANG Sheng¹, BAO Yue-Yue¹, LIU An-Qi¹, ZHUANG Jing-Jing¹, JIANG Biao², LIU Wei^1,*

(1. School of Plant Protection, Anhui Agricultural University, Hefei 230036, China; 2. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032, China)

Online:2024-03-20 Published:2024-04-17

摘要/Abstract

摘要： 【目的】研究大蒜E素(allicin E， ALE)对斑翅果蝇Drosophila suzukii产卵行为的影响，为利用ALE生物防治斑翅果蝇提供参考依据。【方法】采用产卵双向选择模型检测0.01%， 0.015%和002% ALE对斑翅果蝇雌成虫产卵驱避作用。利用暗盒实验、摘除前足、摘除触角等方式检测视觉、味觉和嗅觉感觉系统对斑翅果蝇雌成虫产卵偏嗜行为的影响。利用Y生物迷宫试验检测0015% ALE对斑翅果蝇雌成虫的产卵行为驱避作用。通过检测存活率和发育历期研究ALE对斑翅果蝇适合度的影响。利用荧光染色法检测ALE处理后斑翅果蝇成虫肠道中活性氧水平。【结果】 ALE驱避斑翅果蝇雌成虫产卵，对0.01%， 0.015%和0.02% ALE的产卵指数分别为-0.30，-0.44和-0.51。在黑暗和摘除前足条件下，斑翅果蝇雌成虫存在对ALE的显著的产卵避性反应，而摘除触角的斑翅果蝇对ALE的产卵避性显著降低，对0.01%， 0.015%和0.02% ALE的产卵指数分别降低为-0.10，-0.11和-012。斑翅果蝇雌成虫所产卵暴露于0.02% ALE后，后代蛹和成虫的存活率分别降低了6923%和6970%，后代成蛹和羽化时间分别延长5.88和4.75 d。ALE缩短斑翅果蝇成虫寿命，雌性和雄性在0.02% ALE处理下LT₅₀值分别由39 d降低为18和15 d。 002% ALE处理72 h后斑翅果蝇成虫奔跑和攀爬速度显著降低。0.02%和0.05% ALE处理3 d后斑翅果蝇成虫肠道中的活性氧水平上升。【结论】ALE引起斑翅果蝇雌成虫产卵避性，主要通过嗅觉介导此种产卵避性；ALE延缓了斑翅果蝇后代的生长发育，降低了亲代成虫的运动活力和存活率，并引起肠道损伤，因此具有潜在的生物防治斑翅果蝇价值。

关键词: 斑翅果蝇, 大蒜E素, 产卵避性, 嗅觉系统, 活性氧

Abstract: 【Aim】To study the effects of allicin E (ALE) on the oviposition behavior of Drosophila suzukii, and to provide a reference for the biological control of D. suzukii by ALE. 【Methods】The avoidance effects of 0.01%， 0.015% and 0.02% ALE on the oviposition of D. suzukii female adults were detected with oviposition two-choice device. The effects of sensory systems of vision, gustation and olfaction on the oviposition preference of female adults of D. suzukii were investigated by means of darkness, surgically removing the forelegs and antennae, respectively. The avoidance effects of 0.015% ALE on the oviposition behavior of female adults of D. suzukii were tested by Y-maze experiment. The effects of ALE on the fitness of D. suzukii were tested by determining the survival rate and developmental duration. The reactive oxygen species (ROS) level in the intestines of D. suzukii adults treated with ALE was detected by fluorescent staining. 【Results】ALE had avoidance effects on the oviposition of female adults of D. suzukii, and the oviposition indexes to 0.01%, 0.015% and 0.02% ALE were -0.30, -0.44 and -0.51, respectively. In darkness and forelegless groups, the female adults of D. suzukii still showed significant oviposition avoidance to ALE. However, the female adults of D. suzukii with antennae removed had significantly decreased oviposition avoidance to ALE, and the oviposition indexes to 001%, 0.015% and 0.02% ALE were decreased to -0.10, -0.11 and -0.12, respectively. After the eggs laid by female adults of D. suzukii were exposed to 0.02% ALE, the offspring pupal and adult survival rates were decreased by 69.23% and 69.70%, respectively, and the time to puparium formation and time to adult eclosion of offspring were prolonged by 5.88 and 4.75 d, respectively. ALE shortened the adult life span of D. suzukii, and the LT₅₀ values of females and males under 0.02% ALE treatment decreased from 39 d to 18 and 15 d, respectively. The running and climbing speeds of D. suzukii adults were significantly decreased at 72 h after treatment with 0.02% ALE. The ROS levels in the intestine of D. suzukii adults were increased at 3 d after treatment with 0.02% and 0.05% ALE. 【Conclusion】ALE induces oviposition avoidance in female adults of D. suzukii, which is mediated mainly by olfaction. ALE prolongs the growth and development of the offspring of D. suzukii, decreases the locomotion performance and the survival rate of adults, and causes intestine damage in adults, thereby having potential biological control value against D. suzukii.

Key words: Drosophila suzukii; allicin E, oviposition avoidance, olfactory system, reactive oxygen species

李一璇, 李隆琦, 张晟, 鲍悦月, 刘安琪, 庄晶晶, 姜标, 刘威. 大蒜E素调控斑翅果蝇产卵选择及适合度[J]. 昆虫学报, 2024, 67(3): 374-383.

LI Yi-Xuan, LI Long-Qi, ZHANG Sheng, BAO Yue-Yue, LIU An-Qi, ZHUANG Jing-Jing, JIANG Biao, LIU Wei. Allicin E modulates oviposition preference and fitness in Drosophila suzukii (Diptera: Drosophilidae)[J]. Acta Entomologica Sinica, 2024, 67(3): 374-383.

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大蒜E素调控斑翅果蝇产卵选择及适合度

Allicin E modulates oviposition preference and fitness in Drosophila suzukii (Diptera: Drosophilidae)

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