›› 1997, Vol. 40 ›› Issue (-1): 13-19.

• 研究论文 • 上一篇    下一篇

不同地区棉铃虫种群DNA多态性的RAPD分析

雷仲仁 李 莉 郭予元   

  • 出版日期:1997-12-20 发布日期:1997-12-20

RAPD ANALYSIS OF DNA POLYMORPHISMS IN COTTON BOLLWORM (HELICOVERPA ARMIGERA) FROM DIFFERENT REGIONS OF CHINA

Lei Zhongren Li Li Guo Yuyuan   

  • Online:1997-12-20 Published:1997-12-20

摘要: 通过对棉铃虫Helicoverpa armigera(Hubner)17个地理种群DNA多态性进行了RAPD分析,结果表明:棉铃虫可以分为四大类群,三亚(SY)、莎车(SC)和哈密(HM)种群各为独立的一类,与内地种群有明显的差异,其中三亚种群差异最大;第四类包含14个地理种群,它们之间相对分化较小,基因相似程度较高,特别是黄河流域和辽河流域南部的种群间遗传距离最小,无基因交流障碍。单个个体的RAPD分析表明,这些种群间的某些个体相互交错,进一步说明这些种群间可能存在着广泛的基因交流。最后还讨论了形成棉铃虫遗传多态性的主要原因,

关键词: 棉铃虫, 地理种群, 遗传变异, RAPD分析

Abstract: The results of RAPD analysis of genetic variation among 17 geographicpopulations of cotton bollworm (CBW) showed that these populations could be divided into 4 distinct groups. (1)Sanya (SY) group, (2)Shache (3) group, (4)Hami (HM) group and (4)the rest (14 populations). The SY group had the longest genetic distance to the other groups and the 4th group had rather few genetic variations or much more gene similarity among populations. The genetic distance between Huanghe River Valley population and Liaohe River Valley population was the shortest. It was found by individual RAPD analysis that the individuals possessing the same genetic characteristicswere distributed disorderly in different geographic populations, indicating that there are frequent gene exchanges among populations from different ecological regions. Lastly, the reason of variation among geographic populations of CBW was discussed.

Key words: Helicoverpa armigera, geographic populations, genetic variation, RAPD analysis