›› 2015, Vol. 58 ›› Issue (1): 45-52.

• 研究论文 • 上一篇    下一篇

取食Bt棉与玉米的越冬代棉铃虫抗寒性特征比较

曹婧1,2, 欧阳芳2, 戈峰2, 杨中侠1,*, 肖铁光1,*   

  1. (1.湖南农业大学植物保护学院, 湖南长沙 410128; 2. 中国科学院动物研究所, 农业虫害鼠害综合治理研究国家重点实验室, 北京 100101)
  • 出版日期:2015-01-20 发布日期:2015-01-20
  • 作者简介:曹婧, 女, 1989年2月生, 湖南株洲人, 硕士研究生, 研究方向为农业昆虫与害虫防治, E-mail: caojing_12@163.com

Hardiness characteristics of the overwintering generation of Helicoverpa armigera (Lepidoptera: Noctuidae) feeding on Bt cotton and corn

CAO Jing1, 2 , OUYANG Fang2, GE Feng2, YANG Zhong-Xia1, *, XIAO Tie-Guang1, *   

  1. (1. College of Plant Protection, Hunan Agricultural University, Changsha 410128, China; 2.State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China)
  • Online:2015-01-20 Published:2015-01-20

摘要: 【目的】针对由Bt棉花和玉米构成的华北农田景观, 探讨不同寄主作物对棉铃虫 Helicoverpa armigera 越冬抗寒的影响。【方法】2013年在河北廊坊科研中试基地的作物田中将棉铃虫幼虫接到Bt棉和玉米上, 比较取食不同作物后棉铃虫的化蛹率、存活率和越冬蛹羽化率;在室内控制条件下分别用棉蕾和鲜玉米粒饲喂棉铃虫幼虫。 测定滞育蛹和非滞育蛹的鲜重、干重、以及脂肪、糖原和低分子物质含量等指标, 比较取食不同作物后棉铃虫的抗寒能力。【结果】取食玉米的棉铃虫滞育蛹干重(117.5 mg)、脂肪含量(457.2 μg/mg DW)以及海藻糖浓度(86.45 μg/g)均显著高于取食Bt棉的棉铃虫滞育蛹干重(56.6 mg)、脂肪含量(239.6 μg/mg DW)以及海藻糖浓度(13.87 μg/g);取食玉米的棉铃虫冰点(-10.2℃)显著低于取食Bt棉的棉铃虫冰点(-6.5℃)。【结论】结果表明取食玉米更加有利于棉铃虫越冬。 据历史数据, 近年来玉米种植面积不断增加, 这将提高棉铃虫成功越冬比率, 对棉铃虫种群扩张起到促进作用, 因此注重玉米上棉铃虫的防治尤为重要。

关键词: 棉铃虫, Bt棉花, 玉米, 滞育蛹, 非滞育蛹, 抗寒性, 海藻糖

Abstract: 【Aim】 From the perspective of the farmland landscape in north China composed of Bt cotton and corn, the research aims to explore the influence of different hosts on hardiness of the overwintering generation of  Helicoverpa armigera. 【Methods】 H. armigera  larvae were inoculated on Bt cotton and corn in the field of scientific base in Langfang, Heibei, 2013. During this period, the pupation rate, diapause rate and emergence rate of overwintering pupae of H.armigera feeding on different types of crops were detected and compared. Indoors, H. armigera  larvae were fed on buds of cotton and fresh corn kernels and then the hardiness of diapausing pupae and non-diapausing pupae were compared in terms of the fresh weight, dry weight, fat content, glycogen content, and contents of low molecular substances. 【Results】 It was found that the diapausing pupae of  H. armigera  feeding on corn had obviously higher dry weight(117.5 mg vs 56.6 mg), fat content (457.2 μg/mg DW vs 239.6 μg/mg DW) and trehalose content(86.45 μg/g vs 13.87 μg/g), and lower freezing point (-10.2℃ vs -6.5℃) than the diapausing pupae feeding on Bt cotton (P<0.05). 【Conclusion】 The results indicate that corn is the ideal host for H. armigera to live through the winter in the field. According to the historical data, the planting area of corn has been increasing in recent years, which will facilitate the successful overwintering and population expansion of H. armigera. Therefore, it is significant to control H. armigera in corn field.

Key words: Helicoverpa armigera, transgenic cotton, corn, diapausing pupae, nondiapausing pupae, hardiness, trehalose