昆虫学报 ›› 2020, Vol. 63 ›› Issue (5): 523-532.doi: 10.16380/j.kcxb.2020.05.001

• 研究论文 •    下一篇

草地贪夜蛾雄性成虫和5龄幼虫的转录组比较分析

廖永林1,#, 李燕芳1,#, 杨斌3, 赵华4, 高燕1陈伟平1, 蔡时可2, 王继华2,*   

  1. (1. 广东省农业科学院植物保护研究所, 广东省植物保护新技术重点实验室, 广州 510640; 2. 广东省农业科学院作物研究所, 广东省农作物遗传改良重点实验室, 广州 510640; 3. 中国农业科学院植物保护研究所, 北京 100193; 4. 广东省农业技术推广总站, 广州 510500)
  • 出版日期:2020-05-20 发布日期:2020-06-08

Comparative analysis of the transcriptomes of male adult and the 5th instar larva of Spodoptera frugiperda (Lepidoptera: Noctuidae)

LIAO Yong-Lin1,#, LI Yan-Fang1,#, YANG Bin3, ZHAO Hua4, GAO Yan1, CHEN Wei-Ping1, CAI Shi-Ke2, WANG Ji-Hua2,*    

  1. (1. Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 2. Guangdong Key Laboratory for Crops Genetic Improvement, Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; 3. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 4. Guangdong Province General Station for Agriculture Technology Extension, Guangzhou 510050, China)
  • Online:2020-05-20 Published:2020-06-08

摘要: 【目的】草地贪夜蛾Spodoptera frugiperda是一种新近入侵我国的重要害虫。本研究旨在对草地贪夜蛾雄性成虫和5龄幼虫两个不同发育阶段的转录组进行比较分析。【方法】利用高通量测序技术对草地贪夜蛾雄性成虫和5龄幼虫进行转录组测序和数据组装,并对转录组数据进行功能注释和比较分析。【结果】经de novo组装共获得209 002条转录本,平均长度为687.55 bp,N50为982 bp。共有46 198条(57.43%) unigene在至少一个数据库中获得功能注释,其中1 713条(2.13%) unigene在所有数据库中均能获得注释。在GO数据库中获得205 269条unigene的注释,主要包括68个功能分类;在KEGG数据库中共有3 408条unigene得到注释,涉及277个代谢通路。共鉴定到424个嗅觉相关的基因,并且在雄性成虫和5龄幼虫之间的表达存在差异。通过比较转录组分析,在雄性成虫中鉴定到9 162个上调和6 399个下调差异表达基因(DEGs);功能富集分析发现在上调DEGs中涉及信息素以及信号转导的代谢通路显著富集,而下调DEGs中涉及解毒相关的通路显著富集。【结论】这些转录组数据为探究草地贪夜蛾的生长发育、嗅觉相关功能基因以及候选分子靶标提供资源信息。

关键词: 草地贪夜蛾, 发育阶段, 嗅觉基因, 转录组, 基因注释, 生物信息学

Abstract: 【Aim】 The fall armyworm, Spodoptera frugiperda, is a newly invaded important pest in China. This study aims to comparatively analyze the transcriptomes of two developmental stages, male adult and the 5th instar larva, of S. frugiperda. 【Methods】 High-throughput sequencing technology was used to sequence and assembly the transcriptomes of male adult and the 5th instar larva of S. frugiperda, and the functional annotation and comparative analysis of transcriptome data were performed. 【Results】 After a de novo assembly, a total of 209 002 transcripts were obtained, with an average length of 687.55 bp and an N50 of 982 bp. A total of 46 198 (57.43%) unigenes were annotated in at least one database, among which 1 713 (2.13%) unigenes were annotated in all databases. In the GO database, 205 269 unigene annotations were obtained, mainly including 68 functional classifications. A total of 3 408 unigenes were annotated in KEGG database, involving 277 metabolic pathways. A total of 424 olfaction-related genes were identified and showed differential expression in male adults and the 5th instar larvae of S. frugiperda. By comparative transcriptome analysis, 9 162 up-regulated differentially expressed genes (DEGs) and 6 399 down-regulated DEGs were identified in male adult. Functional enrichment analysis revealed that the up-regulated DEGs were significantly enriched in the pathways involved in pheromone and signal transduction, while the down-regulated DEGs were significantly enriched in the pathways related to detoxification. 【Conclusion】 These transcriptome data provide some valuable information for exploring the functional genes related to the growth and development and olfaction and candidate molecular targets in S. frugiperda.

Key words: Spodoptera frugiperda, developmental stage, olfactory gene, transcriptome, gene annotation, bioinformatics