昆虫学报 ›› 2021, Vol. 64 ›› Issue (9): 1112-1119.doi: 10.16380/j.kcxb.2021.09.011

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华北大黑鳃金龟对桃树挥发物的电生理反应

张诺1,2, 陈立2, 谢广林1,*   

  1.  (1. 长江大学农学院, 湖北荆州434025; 2. 河北大学生命科学学院, 生命科学与绿色发展研究院, 河北保定071002)
  • 出版日期:2021-09-20 发布日期:2021-09-03

Electrophysiological responses of Holotrichia oblita (Coleoptera: Melolonthidae) to peach tree volatiles 

ZHANG Nuo1,2, CHEN Li2, XIE Guang-Lin1,*   

  1.  (1. School of Agriculture, Yangtze University, Jingzhou, Hubei 434025, China; 2. College of Life Science, Institute of Life Science and Green Development, Hebei University, Baoding, Hebei 071002, China)
  • Online:2021-09-20 Published:2021-09-03

摘要:

【目的】华北大黑鳃金龟Holotrichia oblita是一种分布范围广,寄主种类多的农业害虫。本研究鉴定了桃树顶空挥发物中对华北大黑鳃金龟雌虫具有触角电生理活性的化合物,并探索桃树挥发物活性成分的剂量对华北大黑鳃金龟雌、雄虫触角电生理反应的影响。【方法】采用顶空吸附法收集桃树枝叶挥发物,用气相色谱触角电位联用技术(GC-EAD)和气相色谱质谱联用技术(GC-MS)鉴定有电生理活性的桃树挥发物成分,最后用触角电位(electroantennograph, EAG)技术测试这些挥发物在0.01,0.1, 1, 10和100 μg剂量下引起的华北大黑鳃金龟雌、雄成虫EAG反应。【结果】桃树挥发物中能引起华北大黑鳃金龟雌成虫触角电生理反应的成分有7种,包括Z-β-罗勒烯、顺-3-己烯乙酸酯、顺-3-己烯醇、壬醛、水杨酸甲酯以及2种未知化合物。所测试的对华北大黑鳃金龟雌成虫触角具有电生理活性的5种桃树挥发物成分在各剂量下都能引起雌、雄成虫明显的EAG反应。雌、雄虫对同种物质的EAG反应随着剂量的增加而有增大的趋势,并且雌虫的EAG反应显著高于雄虫。【结论】桃树挥发物中,罗勒烯、顺-3-己烯乙酸酯、顺-3-己烯醇、壬醛、水杨酸甲酯均能引起华北大黑鳃金龟雌、雄成虫的剂量依赖性的触角电生理反应,而且存在显著的性别差异。可利用这些化合物开展行为反应和田间诱集试验,进一步验证其对华北大黑鳃金龟的引诱活性。

关键词: 华北大黑鳃金龟, 桃树, 植物挥发物, 剂量, 气相色谱-触角电位联用技术, 触角电位技术

Abstract:  【Aim】 Holotrichia oblita is an economically important agricultural pest with a wide range of distribution and various host plant species. This study aims to identify peach tree volatiles that have the electrophysiological activity to the antennae of the female adults of H. oblita, and to further explore the effect of doses of the identified volatiles on the electrophysiological response in female and male antennae. 【Methods】 Volatiles from peach tree leaves were collected by dynamic headspace adsorption. The electrophysiologically active compounds from peach tree volatiles were identified by gas chromatography-electroantennographic detection (GC-EAD) and gas chromatography-mass spectrometry (GC-MS) techniques, and the responses of the antennae of female and male adults of H. oblita to the identified volatile compounds at doses of 0.01, 0.1, 1, 10 and 100 μg were tested with electroantennogram (EAG) technique. 【Results】 There were seven compounds in the peach tree volatiles that could trigger the electrophysiological reaction in the antennae of female adults of H. oblita, including Z-β-ocimene, Z-3-hexenyl acetate, Z-3-hexanol, nonanal, methyl salicylate and two unknown compounds. All the five peach tree volatile compounds with the electrophysiological activity to the antennae of female adults of H. oblita could trigger distinct EAG responses at all test doses in both female and male adults of H. oblita. The EAG responses of females and males to the same compound tended to increase with the increase of dose. The EAG response of females was significantly higher than that of males.  【Conclusion】 H. oblita adults generally show significant dose-dependent electroantennographic responses to Z-β-ocimene, Z-3-hexenyl acetate, Z-3-hexanol, nonanal, and methyl salicylate. There exists significant sexual difference in the EAG response. These compounds can be used for behavioral response and field trapping experiments to further confirm their attractiveness to H. oblita.

Key words: Holotrichia oblita, Prunus persica, plant volatile, dose, gas chromatography-electroantennographic detection, electroantennography