›› 1997, Vol. 40 ›› Issue (1): 7-14.

• 研究论文 • 上一篇    下一篇

混合感染后杆状病毒间的增效作用——病毒蛋白及核酸的初步分析

王燕 丁翠 蔡秀玉   

  • 出版日期:1997-02-20 发布日期:1997-02-20

THE SYNERGISTIC ACTION BETWEEN BACULOVIRUSESAFTER MIXED INFECTION--A PRIMARY ANALYSIS OF VIRAL PROTEINSAND NUCLEIC ACIDS

Wang Yan Ding Cui Cai Xiuyu   

  • Online:1997-02-20 Published:1997-02-20

摘要: AsNPV+HasNPV、AsNPV+HaNPV、AsNPV+PsNPV分别感染烟青虫、棉铃虫和粘虫幼虫,对分离到的核型多角体病毒(AsNPV+HasNPV)-Helicoverpa assulta、(AsNPV+HaNPV)- H.Armigera和(AsNPV+PsNPV)-Pseudaletia separata,经电镜观察,多角体蛋白及病毒粒子蛋白SDS-PAGE电泳,病毒核酸的限制性内切酶酶解分析等研究,证明各病毒的多角体形态不规则,大小差异极大,病毒粒子为杆状,(AsNPV+HasNPV)-H.Assulta 和(AsNPV+HaNPV)-H.Armigcra病毒粒子有单粒和多粒包埋类型, (AsNPV+PsNPV)-P.Separata为多粒包埋型。各病毒的多角体蛋白基本上只有一种多肽,分子量为25 000道尔顿左右。(AsNPV+HasNPV)-H.Assulta、(AsNPV+HaNPV)-H.armigera和(AsNPV+PsNPV)-P.Separata的病毒粒子分别有10、14、5条多肽,分子量大小在13 500~98 000,13 000~88 000,18 500~52 000道尔顿之间。病毒核酸经EcoRI,HindIII,HindIII+BamHI酶解,其DNA的酶切位点,大小及DNA的总分子量与AsNPV和原寄主的Has-NPV,HaNPV和PsNPVDNA的酶切图谱存在一定差异,混合病毒侵染昆虫后新复制的病毒核酸发生一定的变化,从而导致病毒蛋白和病毒形态的变化。混合感染后AsNPV对Has-NPV、HaNPV和PsNPV的侵染有明显的增效作用,其机理有待深入研究。

关键词: 核型多角体病毒, 黄地老虎, 烟青虫, 棉铃虫, 粘虫, 混合侵染, 增效

Abstract: AsNPV was mixed with HasNPV, HaNPV and PsNPV separately and thethree mixtures were used to infect the larvae of Helicoverpa assulta, H. arrnigera and Pseudaletia separata respectively. The nuclear polyhedrosis viruses thus obtained were called ( AsNPV+HasNPV) -H. assulta, (AsNPV+HaNPV) -H. armigera and (AsNPV+PsNPV) -P. separata respectively. The polyhedra of the NPVs were in irregular shape and varied distinctly in sizes. The biochemical charcaters of the viruses were investigated andcompared with those of HasNPV, HaNPV and PsNPV. The polyhedrin and viral structural polypeptides of the NPVs were examined by using SDS-PAGE. The polyhedrin of all NPVs had molecular weight of about 25 000 dalton. The numbers of the viral structural polypeptides of (AsNPV+HasNPV)-H. assulta, (AsNPV+HaNPV)-H. armigera and (AsNPV+PsNPV) -P. separata were 10, 14, 5 respectively, and their molecular weights were 13 500-98 000, 13 000-88 000, 18 500-52 000 dalton. The analysis of agarose electrophoretic profiles of DNAs restriction endonuclease fragments showed that they were notidentical to those of AsNPV, HasNPV, HaNPV and PsNPV. The DNAs of the viruses had changed and resulted in the changes of viral proteins and viral morphogenesis. Agrotis segetum nuclear polyhedrosis virus (AsNPV) can enhance the infection of HasNPV, HaNPV and PsNPV. The enhancement mechanism of AsNPV is worthwhile to be further investigated.

Key words: nuclear polyhedrosis virus (NPV), Agrotis segetum, Helicoverpa assulta, He- licoverpa armigera , Pseudaletia separata , mixed infection, enhancement