Acta Entomologica Sinica ›› 2023, Vol. 66 ›› Issue (6): 825-834.doi: 10.16380/j.kcxb.2023.06.011

• RESEARCH PAPERS • Previous Articles     Next Articles

Sequencing and analysis of the complete mitochondrial genome of Campsiura mirabilis (Coleoptera: Scarabaeidae)

HU Li, YANG Lin, TIAN Tian, CHEN Bin*   

  1.  (Chongqing Key Laboratory of Vector Insects, Institute of Entomology and Molecular Biology, Chongqing Normal University, Chongqing 401331, China)
  • Online:2023-06-20 Published:2023-08-02

Abstract: 【Aim】 To sequence and analyze the complete mitochondrial genome (mtgemome) of Campsiura mirabilis, and to discuss the phylogenetic relationship of the family Scarabaeidae based on mtgenome sequences. 【Methods】 The whole mtgenome of C. mirabilis was firstly sequenced on the Illumina Hiseq X Ten platform, and the gene structure characteristics and base composition of the C. mirabilis mtgenome were analyzed. Combined with the 54 mtgemome sequences published in GenBank, the selection pressure of protein-coding genes (PCGs) in the mtgenome of 55 species in Scarabaeidae was analyzed. The phylogenetic tree of Scarabaeidae was constructed with Lucanus mazama in Lucanidae as the outgroup based on PCG and rRNA gene sequences using maximum likelihood (ML) and Bayesian inference (BI) methods. 【Results】 The full-length of the C. mirabilis mtgenome (GenBank accession no.: MT548771) is 16 123 bp, including 13 PCGs, 22 tRNA genes, 2 rRNA genes and a Dloop region (control region), without gene rearrangement. All the 22 tRNA genes show the typical cloverleaf secondary structure except for the absence of DHU arm of trnS1. The base composition of the mtgenome of C. mirabilis shows a higher AT content with the positive AT-skew and negative GC-skew. The Ka/Ks values of PCGs of Scarabaeidae are all less than 1, indicating that all of these 13 PCGs have experienced purifying selection. The phylogenetic relationship in Scarabaeidae based on the sequences of the 13 PCGs and 2 rRNA genes was: ((Aphodiinae+Scarabaeinae)+(Melolonthinae+(Cetoniinae+(Rutelinae+Dynastinae)))). 【Conclusion】 In this study we sequenced and analyzed the mtgenome of C. mirabili, and analyzed the phylogenetic relationship of Scarabaeidae. Our results indicate that the subfamilies of Scarabaeinae, Cetoniinae, Rutelinae, Dynastinae and Melolonthinae are monophyletic groups.

Key words: Campsiura mirabilis, Scarabaeidae, Cetoniinae, mitochondrial genome, phylogenetic relationship