Acta Entomologica Sinica ›› 2023, Vol. 66 ›› Issue (9): 1233-1245.doi: 10.16380/j.kcxb.2023.09.010

• RESEARCH PAPERS • Previous Articles     Next Articles

Sequencing and analysis of the complete mitochondrial genome of Eurytoma acutibialis (Hymenoptera: Eurytomidae), an important natural enemy insect of Bradybatus sp. (Coleoptera: Curculionidae) in China

LIU Hui-Hui1,*, LI En-Jie1, CAO Liang-Ming2, BAO Qing-Chun3, WANG Xiao-Lin3, XIN Xue-Bing1, YANG Zhong-Qi2,*   

  1.  (1. National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 102300, China; 2. Key Laboratory of Forest Protection of National Forestry and Grassland Administration, Ecology and Nature Conservation Institute, Chinese Academy of Forestry, Beijing 100091, China; 3. Wujiaofeng Natural Reserve of Horqinyouyizhongqi, Xing′an League 137400, China)
  • Online:2023-09-20 Published:2023-09-27

Abstract: 【Aim】 Eurytoma acutibialis Yang, Liu et Cao is a parasitic wasp of Bradybatus sp., a serious seed-eating pest of Acer truncatum in China, and is a new species found during the investigation of Bradybatus sp. and can play an important role in controlling Bradybatus sp. To further understand the new parasitoid, the complete mitochondrial genome of E. acutibialis was sequenced and analyzed so as to provide molecular biological evidence for the systematic taxonomy and phylogenetic relationship of hymenopterous wasps (Hymenoptera: Chalcidoidea: Eurytomidae).【Methods】 The complete mitochondrial genome of E. acutibialis was sequenced on Illumina HiSeqTM4000 sequencing platform and then assembled, annotated and analyzed. Based on the nucleotide sequences of 13 protein-coding genes (PCGs) from 40 species of hymenopteran insects in the Chalcidoidea, the phylogenetic tree was constructed by Bayesian inference (BI) and maximum likelihood (ML) methods, and the phylogenetic relationship between E. acutibialis and other insects in the Chalcidoidea was analyzed.【Results】The mitochondrial genome of E. acutibialis is 15 849 bp in full-length, with the A+T content of 82.00%, and consists of 37 genes including 13 PCGs, 2 rRNA genes, 22 tRNA genes, and a control region (CR). Among the 13 PCGs, the amino acid usage frequencies from high to low are Leu,Ile, Met, Phe and Asn. All the 13 PCGs initiate with the standard start codons of ATN (ATT, ATG and ATA) and terminate with the typical stop codons TAA/TAG. Among the 22 tRNA genes, trnI lacks receptor arm, trnF lacks TΨC-arm, trnS1 lacks DHU arm and DHU arm of trnS2 forms a simple loop. The remaining tRNA genes have a typical cloverleaf structure. There is a significant gene rearrangement, with an inversion occurring in the nad2-nad1-cytb-nad6-nad4L-nad4-nad5 cluster and rearrangements of 16 tRNA genes. The phylogenetic analysis highly supported the monophyly of the subfamily Eurytomidae, and E. acutibialis was the closest to Eurytoma sp. ZJUH 2016013.【Conclusion】The complete mitochondrial genome sequence of E. acutibialis was firstly obtained, and the phylogenetic analysis based on the mitochondrial genome supported its taxonomic position in the genus Eurytoma in the family Eurytomidae in the order Hymenoptera and proved to be consistent with the morphological taxonomy.

Key words:  Eurytomidae; Eurytoma acutibialis; Bradybatus sp., mitochondrial genome, gene rearrangement