›› 1999, Vol. 42 ›› Issue (2): 113-119.

• RESEARCH PAPERS •     Next Articles

Inhibition of protein phosphorylation and ATPase activity by deltamethrin in housefly brain synaptosomes

Tian Yu, Leng Xin-fu   

  • Online:1999-05-20 Published:1999-05-20

Abstract: Deltamethrin was found to affect ATPase activity and protein phosphorylation of housefly brain synaptosome in vitro. Both Na, K-ATPase and Ca-ATPase activities can be inhibited by various concentrations of deltamethrin (10-9~10-4mol/L). However, the inhibition was higher in susceptible housefly than in deltamethrin resistant strain. In intact susceptible housefly brain synaptosome the most significant result was inhibition of endogenous protein phosphorylation by 10-5 mol/L of deltamethrin. Such effect in deltamethrin resistant housefly was suppressed by adding the exogenous Ca2+ or Ca2+ and calmodolin to the reaction system.Cyclic AMP stimulated the overall phosphorylation level,particularly 48 kD protein in susceptible housefly. The 48 kD protein,isoelectric point pI 5.2,may be the brain-specific B-50 protein in housefly.All these results indicated that: (1)Deltamethrin has the property to affect ATPase activity and protein phosphorylation. (2) Deltamethrin might limits calcium transport which connects with the protein phosphorylation.

Key words: deltamethrin, synaptosome of housefly brain, protein phosphorylation, Na/K-ATPase, Ca-ATPase, deltamethrin-resistant and susceptible strains of Musca domestica