›› 2005, Vol. 48 ›› Issue (1): 139-142.
• RESEARCH PAPERS • Previous Articles Next Articles
AN Chun-Ju, LI De-Sen, ZHAO Su-Ran, DU Rong-Qian*
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Abstract: The influences of gelfiltration chromatography media, column diameter, column height and flow rate on separation performance of the protein crude extracts of housefly (Musca domestica) were investigated.The results indicated that the all above four factors could affect the separation performance of the crude extracts in gelfiltration chromatography by various degrees.In the scope of the present tests, the best separation performance was achieved using Sephadex G-75 (1.3 cm×40 cm) with 0.4 mL/min rate.
Key words: Musca domestica, protein crude extracts, gelfiltration chromatography, separation
AN Chun-Ju, LI De-Sen, ZHAO Su-Ran, DU Rong-Qian*. Optimization of the gelfiltration chromatography parameters for separating proteins from the crude extracts of housefly[J]., 2005, 48(1): 139-142.
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