基于光学显微镜和扫描电镜的新替库蠓成虫形态观察(双翅目： 蠓科)(英文)

doi:10.16380/j.kcxb.2019.09.010

摘要/Abstract

摘要：

【目的】通过发现更多的形态特征，以实现库蠓属Culicoides近似种的准确鉴定和区分。【方法】利用扫描电镜和光学显微镜观察新替库蠓Culicoides newsteadi Austen成虫的形态特征细节，包括具有重要鉴定意义的结构特征，如两性成虫的复眼、触角、触须、口器、中胸盾板、小盾板、翅、后足胫节、爪和生殖器等结构。【结果】发现新替库蠓雌成虫具有6种触角感器，包括腔锥感器、短毛形感器、长毛形感器、刺形感器、直锥形感器和弯直锥形感器；雌雄成虫的盾斑均呈卵形；雄成虫的阳基侧突端部具复杂分支。雌成虫的复眼连接处距离为1.3个小眼面；雌成虫大颚呈剑状，具14~17枚三角形齿；雄成虫后足胫节具16~19枚端梳。【结论】通过光学显微镜和扫描电镜获得的形态特征不仅为蠓科的分类和系统发育研究提供更多的信息，而且为流行病研究和疾控工作提供更多的帮助，进而为蠓科昆虫形态信息的数字化、立体化和网络化提供资料。

关键词: 吸血蠓, 灰黑库蠓复组, 形态, 近似种, 媒介

Abstract:

【Aim】 The aim of this study is to discover the morphological details for accurate identification and differentiation of Culicoides species. 【Methods】 The morphological details of adult Culicoides newsteadi Austen were observed under light microscopy (LM) and scanning electron microscopy (SEM). Important diagnostic features of the compound eyes, antennae, maxillary palpus, mouthparts, mesonotum, scutellum, wing, hind tibia, claws and the genitalia of both females and males were described. 【Results】 Six types of antennal sensilla were found in adult females of C. newsteadi including sensilla coeloconica, short sensilla trichodea, long sensilla trichodea, sensilla chaetica, straight sensilla basiconica, and curved sensilla basiconica scattered on antennal surface. Scutal areolae in adult females and males are ovoid. The tips of the parameres in adult males have complicated branches. Compound eyes in adult females have a short distance about 1.3 facets at the joint. The mandible in adult females has 14-17 triangular teeth. The hind tibial comb in adult females has 16-19 combs. 【Conclusion】 The morphological features obtained by LM and SEM can provide information not only for taxonomy and phylogeny of Ceratopogonidae, but also aid in epidemiology studies and disease control, as well as in the digitization, three-dimensional analysis and networking of morphological information of Ceratopogonidae.

Key words: Biting midges, Pulicaris group, morphology, similar species, vector

蒋晓红, 常琼琼, 段琛, 侯晓晖. 基于光学显微镜和扫描电镜的新替库蠓成虫形态观察(双翅目：蠓科)(英文)[J]. 昆虫学报, 2019, 62(9): 1090-1101.

JIANG Xiao-Hong, CHANG Qiong-Qiong, DUAN Chen, HOU Xiao-Hui. A morphological investigation of adult Culicoides newsteadi Austen (Diptera, Ceratopogonidae) using light microscopy and scanning electron microscopy (In English)[J]. Acta Entomologica Sinica, 2019, 62(9): 1090-1101.

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