褐飞虱成虫不同密度和取食时长胁迫下水稻植株的生理生化响应

doi:10.16380/j.kcxb.2023.02.004

摘要/Abstract

摘要： 【目的】探索褐飞虱Nilaparvata lugens不同密度与取食时长胁迫下水稻植株生理生化响应。【方法】在褐飞虱成虫3个密度(2，4和8头/株)与4个取食时长(6, 24, 48和96 h)的组合处理后，应用生物化学分析方法检测水稻植株中叶绿素(chlorophyll, Chl)与总蛋白(total protein, TP)含量，脂氧合酶(lipoxygenase, LOX)、总超氧化物歧化酶(total superoxide dismutase, T-SOD)、过氧化物酶(peroxidase, POD)与多酚氧化酶(polyphenol oxidase, PPO)活性。【结果】与对照组（未接种褐飞虱成虫）相比，取食6 h时，低密度(2头/株)褐飞虱成虫引起水稻植株中Chl含量显著降低，TP含量和PPO活性显著升高；高密度(8头/株)褐飞虱成虫引起水稻植株中Chlb和总Chl含量、TP含量及LOX和POD活性显著升高。与对照组相比，取食24 h时，低密度褐飞虱成虫引起水稻植株中Chl含量和PPO活性显著降低， LOX, T-SOD和POD活性显著升高，高密度褐飞虱成虫引起水稻植株中Chl含量和PPO活性显著升高，T-SOD和POD活性显著降低。与对照组相比，褐飞虱成虫取食48 h时，低和中(4头/株)密度组水稻植株中Chl含量及T-SOD和POD活性显著降低, TP含量显著升高；取食96 h时，高密度组水稻植株中Chl含量和T-SOD活性显著降低，TP含量和POD活性显著升高。【结论】褐飞虱成虫不同密度与不同时长的取食胁迫均引起水稻植株中Chl与TP含量，及LOX, PPO, T-SOD和POD活性的变化，变化强度与响应时间因褐飞虱成虫取食密度不同有明显差异。

关键词: 褐飞虱, 取食胁迫, 水稻, 诱导反应, 酶活性

Abstract: 【Aim】The purpose of this study is to explore the physiological and biochemical responses in rice plants to the stress imposed by different densities of the adult brown planthopper (BPH), Nilaparvata lugens for different time.【Methods】The contents of chlorophyll (Chl) and total protein (TP), and the activities of lipoxygenase (LOX), total superoxide dismutase (T-SOD), peroxidase (POD) and polyphenol oxidase (PPO) in rice plants fed by N. lugens adults at the densities of 2, 4 and 8 individuals/plant for 6, 24, 48 and 96 h were tested by biochemical methods.【Results】At 6 h after being fed by N. lugens adults at the low density (2 individuals/plant), the Chl content in rice plants decreased significantly and the TP content and PPO activity in rice plants increased significantly, while the Chlb and total Chl contents, TP content and activities of LOX and POD in rice plants at 6 h after being fed by N.lugens adults at the high density (8 individuals/plant) increased significantly as compared to those in the control group non-inoculated with N. lugens adults. Compared to the control group, the stress imposed by low density of N. lugens adults resulted in significantly reduced Chl content and PPO activity, and significantly enhanced activities of LOX, T-SOD and POD in rice plants, and that imposed by high density of N. lugens adults caused significantly increased Chl content and PPO activity, and significantly decreased activities of T-SOD and POD in rice plants at 24 h. In rice plants fed by N. lugens adults at the low density and medium density (4 individuals/plant), the Chl content and activities of T-SOD and POD decreased significantly, and the TP content significantly enhanced at 48 h, while in rice plants fed by N. lugens adults at the high density, the Chl content and T-SOD activity significantly reduced and the TP content and POD activity increased significantly at 96 h, as compared to those in the control group. 【Conclusion】The stress imposed by different densities of N. lugens adults for different time induces changes in the contents of Chl and TP, and activities of LOX, PPO, T-SOD and POD. The change intensity and response time are obviously different due to the feeding density of N. lugens adults.

Key words: Nilaparvata lugens, feeding stress; Oryza sativa, induced reaction, enzyme activity

赵文华, 杨光梅, 刘雨芳. 褐飞虱成虫不同密度和取食时长胁迫下水稻植株的生理生化响应[J]. 昆虫学报, 2023, 66(2): 150-157.

ZHAO Wen-Hua, YANG Guang-Mei, LIU Yu-Fang. Physiological and biochemical responses of rice plants to the stress imposed by different densities of the adult brown planthopper, Nilaparvata lugens(Hemiptera:Delphacidae) for different time[J]. Acta Entomologica Sinica, 2023, 66(2): 150-157.

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褐飞虱成虫不同密度和取食时长胁迫下水稻植株的生理生化响应

Physiological and biochemical responses of rice plants to the stress imposed by different densities of the adult brown planthopper, Nilaparvata lugens(Hemiptera:Delphacidae) for different time

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