两种榈蝽的线粒体全基因组测序及榈蝽科系统发育地位分析

doi:10.16380/j.kcxb.2024.10.011

昆虫学报 ›› 2024, Vol. 67 ›› Issue (10): 1416-1427.doi: 10.16380/j.kcxb.2024.10.011

两种榈蝽的线粒体全基因组测序及榈蝽科系统发育地位分析

张丹丽¹, 陈小艳¹, 袁娟娟^2,*, 杨焕焕³

(1. 太原师范学院生物科学与技术学院, 晋中 030619; 2. 枣庄学院生命科学学院, 枣庄 277160; 3. 齐鲁工业大学(山东省科学院)生物工程学院, 济南 250353)

出版日期:2024-10-20 发布日期:2024-11-18

Sequencing of the complete mitochondrial genomes of two palm bugs and analysis of the phylogenetic position of Thaumastocoridae (Hemiptera: Heteroptera)

ZHANG Dan-Li¹, CHEN Xiao-Yan¹, YUAN Juan-Juan^2,*, YANG Huan-Huan³

(1. College of Biological Sciences and Technology, Taiyuan Normal University, Jinzhong 030619, China; 2. College of Life Sciences, Zaozhuang University, Zaozhuang 277160, China; 3. School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China)

Online:2024-10-20 Published:2024-11-18

摘要/Abstract

摘要： 【目的】测定和分析半翅目(Hemiptera)异翅亚目(Heteroptera)榈蝽科(Thaumastocoridae)两种榈蝽Thaumastocoris peregrinus与Onymocoris hackeri的线粒体全基因组序列，并分析榈蝽科在臭虫次目(Cimicomorpha)的系统发育地位。【方法】通过高通量测序获取榈蝽科T. peregrinus与O. hackeri线粒体全基因组和核基因(18S rDNA基因和28S rDNA基因)序列，对其线粒体基因组进行注释分析和比较。选取GenBank中异翅亚目蝎蝽次目(Nepomorpha)、细蝽次目(Leptopodomorpha)和蝽次目(Pentatomomorpha)共9种线粒体全基因组和核基因(18S rDNA基因和28S rDNA基因)序列作为外群，臭虫次目7科24种与榈蝽科2种线粒体基因组和核基因(18S rDNA基因和28S rDNA基因)序列作为内群，基于13个蛋白质编码基因、 18S rDNA基因和28S rDNA基因序列矩阵利用最大似然法(maximum likelihood，ML)和贝叶斯法(Bayesian inference，BI)构建系统发育树。【结果】T. peregrinus与O. hackeri的线粒体全基因组分别长15 399和15 490 bp，包括13个蛋白质编码基因、 22个tRNA基因、 2个rRNA基因和1个控制区。核苷酸组成存在明显的AT偏好性，没有发现基因重排现象。22个tRNA基因中除了tRNA-Ser(GCU)均可以折叠成经典的三叶草二级结构。控制区均发现了串联重复区域和茎环结构。基于最大似然法和贝叶斯法构建的系统发育关系为：(猎蝽总科(Reduvioidea)+((臭虫总科(Cimicoidea)+(捷蝽总科(Velocipedoidea)+姬蝽总科(Nabioidea)))+盲蝽总科(Miroidea)))。榈蝽科位于盲蝽总科内，形成了(榈蝽科(Thaumastocoridae)+(盲蝽科(Miridae)+网蝽科(Tingidae)))的拓扑关系。【结论】本研究测定和分析了榈蝽科2个物种线粒体全基因组序列，并探讨了榈蝽科在臭虫次目中的系统发育地位，补充了榈蝽科昆虫的分子数据，进一步加强了对臭虫次目系统发育和线粒体基因组进化的理解。
关键词:
中图分类号: Q969文献标识码: A文章编号: 04546296(2024)10141612

关键词: 半翅目, 榈蝽科, 线粒体基因组, rRNA, 系统发育

Abstract: 【Aim】To sequence and analyze the complete mitochondrial genomes of two palm bugs, Thaumastocoris peregrinus and Onymocoris hackeri, and analyze the phylogenetic placement of Thaumastocoridae within Cimicomorpha. 【Methods】The sequences of the complete mitochondrial genomes and nuclear genes (18S rDNA gene and 28S rDNA gene) of T. peregrinus and O. hackeri in Thaumastocoridae were obtained by high-throughput sequencing. The mitochondrial genomes were annotated and compared. The sequences of mitochondrial genomes and nuclear genes (18S rDNA gene and 28S rDNA gene) of nine species of Heteroptera including Nepomorpha, Leptopodomorpha and Pentatomomorpha from GenBank were selected as outgroups and the sequences of the mitochondrial genomes and nuclear genes (18S rDNA gene and 28S rDNA gene) of 24 species of seven families from Cimicomorpha and two species from Thaumastocoridae were selected as ingroups. The phylogenetic trees were constructed using maximum likelihood (ML) and Bayesian inference (BI) methods based on the sequences of 13 protein coding genes, 18S rDNA gene and 28S rDNA gene. 【Results】 The complete mitochondrial genomes of T. peregrinus and O. hackeri were 15 399 and 15 490 bp in length, respectively, including 13 protein-coding genes, 22 tRNA genes, two rRNA genes and one control region. There was an obvious AT preference in nucleotide composition and no gene rearrangement was found. All of the 22 tRNA genes except tRNA-Ser (GCU) can be folded into a typical clover-leaf secondary structure. Tandem repeat region and stem-loop were found in the control region. The phylogenetic relationship based on maximum likelihood and Bayesian inference methods was recovered as (Reduvioidea+((Cimicoidea+(Velocipedoidea+Nabioidea))+Miroidea)). Thaumastocoridae was located within Miroidea, forming a topology of (Thaumastocoridae+(Miridae+Tingidae)). 【Conclusion】 In this study two complete mitochondrial genomes of Thaumastocoridae were sequenced and analyzed, and the phylogenetic position of Thaumastocoridae within Cimicomorpha was explored. The molecular data of Thaumastocoridae were supplemented, which further enhanced the understanding of the phylogeny of Cimicomorpha and mitochondrial genome evolution.

Key words: Hemiptera, Thaumastocoridae, mitochondrial genome, rRNA, phylogeny

张丹丽, 陈小艳, 袁娟娟, 杨焕焕. 两种榈蝽的线粒体全基因组测序及榈蝽科系统发育地位分析[J]. 昆虫学报, 2024, 67(10): 1416-1427.

ZHANG Dan-Li, CHEN Xiao-Yan, YUAN Juan-Juan, YANG Huan-Huan. Sequencing of the complete mitochondrial genomes of two palm bugs and analysis of the phylogenetic position of Thaumastocoridae (Hemiptera: Heteroptera)[J]. Acta Entomologica Sinica, 2024, 67(10): 1416-1427.

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两种榈蝽的线粒体全基因组测序及榈蝽科系统发育地位分析

Sequencing of the complete mitochondrial genomes of two palm bugs and analysis of the phylogenetic position of Thaumastocoridae (Hemiptera: Heteroptera)

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