›› 1997, Vol. 40 ›› Issue (-1): 61-65.

• 研究论文 • 上一篇    下一篇

小麦植株挥发性次生物质对棉铃虫的行为及EAG反应

张钟宁 李秀珍 方宇凌 向余劲攻 耿文军   

  • 出版日期:1997-12-20 发布日期:1997-12-20

EFFECT OF VOLATILES OF WHEAT SECONDARY METABOLITES ON THE BEHAVIOUR AND ELECTROANTENNOGRAM OF HELICOVERPA ARMIGERA (HUBNER)

Zhang Zhongning Li Xiuzhen Fang Yuling Xiangyu Jingong Geng Wenjun   

  • Online:1997-12-20 Published:1997-12-20

摘要: 本文用无水乙醚及水蒸汽蒸馏两种方法,分别提取小麦花和叶各1 000g和3 200g。4个提取样品分别用氮气浓缩至2 mL。然后用风洞和触角电位检测棉铃虫Helicoverpaarmigera对小麦花、叶提取物的行为及电生理的反应。风洞实验结果表明:小麦花、叶中所含的挥发性次生物质对棉铃虫有很好的引诱作用,其定向率为45%~72%,高于对照22.2%,经‘检验差异显著;此外,雌蛾对花、叶的定向率均高于雄蛾;而花与叶的提取物对雌、雄蛾的定向率之间无显著差异。触角电位实验结果表明:棉铃虫触角对小麦花、叶的提取物均有强的EAG反应,与对照相比有显著性差异;雌虫的EAG反应略高于雄虫,但差异不显著;小麦花提取物对棉铃虫EAG反应略强于小麦叶,但差异不显著。

关键词: 小麦, 挥发性次生物质, 棉铃虫, 行为, EAG

Abstract: In this paper, the extracts of wheat flowers and wheat leaves (1 000 g and 3 200 g respectively) were drawn forth both by ethyl ether and steam distillation. The four samples were concentrated to two milliliters each by nitrogen. Activity of the extracts attracting cotton bollworm moths was tested by wind-tunnel and electroantennographic responses. In wind tunnel, the result showed that. (1) the volatile secondary metabolites from wheat flowers and wheat leaves possess attractive effect on cotton bollworm moths. The orientation rate were 45% to 72%, much higher than 22.2% of control, with significent difference by t test; (2)the orientation rates of females were higher than those of males; and (3) there were no significant difference between flowers and leaves. On EAG test, the result showed that antenna of cotton bollworm strongly responsed to the extracts of wheat flowers and leaves with significent difference to the control. EAG response of male was slightly higher than that of female, and the EAG response of cotton bollworm to wheat flower extract was slightly higher than to leave extract.

Key words: wheat, volatile secondary metabolites, Helicoverpa armigera (Hubner), behaviour, electroantannogram