Acta Entomologica Sinica ›› 2019, Vol. 62 ›› Issue (8): 895-900.doi: 10.16380/j.kcxb.2019.08.001

• RESEARCH PAPERS •     Next Articles

Cellular location of snoRNA Bm-15 and its interaction with Notch-like receptor gene in the silkworm, Bombyx mori

SHEN Ya-Wen1, MA Tian-Tian2, HUO Chun-Yue2, YANG Zong-Lin2, LU Yi-Ping1, KAN Yun-Chao2, LI Dan-Dan 2,*   

  1.  (1. School of Life Sciences, Zhengzhou University, Zhengzhou 45001, China; 2. Henan Key Laboratory of Insect Biology in Funiu Mountain, Nanyang Normal University, Nanyang, Henan 473061, China)
  • Online:2019-08-20 Published:2019-08-29

Abstract: 【Aim】 In a previous study, we found that a C/D box snoRNA Bm-15 of the silkworm, Bombyx mori, could interact with the Notch-like receptor gene NLR in vitro. This study aims to further investigate the mechanisms of interaction between snoRNA Bm-15 and NLR in B. mori. 【Methods】 The expression profiles of snoRNA Bm-15 and NLR in B. mori at different developmental stages of larva, pupa and adult were detected by real-time quantitative PCR. Meanwhile, the cellular location of snoRNA Bm-15 and NLR was detected by in situ hybridization in BmN4 cells to explore the interaction between them. 【Results】 Expression profiles showed that the expression trends of snoRNA Bm-15 and NLR were consistent during larval development of the silkworm, decreasing first and then increasing, with the lowest expression level in the 2nd instar larvae. During pupation and eclosion, the expression level of snoRNA Bm-15 gradually declined, with the lowest expression level in virgin moths. But NLR showed the lowest expression level in the 1 day-old female pupae and the highest expression level in virgin moths. Cellular localization revealed that snoRNA Bm-15 was not only located in the nucleus of BmN4 cells but also in the cytoplasm, while NLR was distributed mainly in the nucleus with overlap with snoRNA Bm-15. 【Conclusion】 This is the first report that a snoRNA Bm-15 exists in both nucleus and cytoplasm in lepidopteran insects, and its interacting gene NLR is mainly in the nucleus and overlapped with snoRNA Bm-15. snoRNA Bm-15 and NLR have consistent expression patterns in the larval stage of the silkworm, but show opposite expression patterns during pupation and eclosion, suggesting that snoRNA Bm-15 and NLR might have different interaction modes in different developmental stages of the silkworm.

Key words: Bombyx mori, development, snoRNA, Bm-15, expression profile, cellular location