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  • Monthly, Founded in 1950
    Supervisor:Chinese Academy of Sciences
    Sponsor:Institute of Zoology,Chinese Academy of Sciences
    The Entomological Society of China
    Domestic postal code: 2-153
    Foreign issuance code: Q61
    ISSN 0454-6296
    CN 11-1832/Q
Table of Content
20 November 2023, Volume 66 Issue 11
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  • RESEARCH PAPERS
    Cloning and expression profiling of heat shock protein genes AcHsp83a and AcHsp83b from Arma chinensis (Hemiptera: Pentatomidae) and their response to high or low temperature and UV-B stresses
    MENG Jian-Yu, JIN Xin, HE Long-Chun, ZHANG Xue-Xia, YANG Chang-Li, ZHANG Chang-Yu
    2023, 66(11):  1425-1434.  doi:10.16380/j.kcxb.2023.11.001
    Abstract ( 234 )   PDF (1904KB) ( 259 )   PDF(mobile) (1904KB) ( 71 )     
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    【Aim】 To explore the molecular mechanism of the natural enemy insect Arma chinensis in response to high or low temperature and UV-B stresses.【Methods】 The heat shock protein genes AcHsp83a and AcHsp83b of A. chinensis were cloned by RT-PCR, and their sequence characteristics were analyzed by bioinformatics methods. RT-qPCR was used to detect the expression levels of AcHsp83a and AcHsp83b in different developmental stages (egg, 1st-5th instar nymphs, female adult and male adult), different adult tissues (head, thorax, abdomen, wing, antenna, fat body, leg, Malpighian tubules, mouthparts, midgut, ovary and testis), and female and male adults at 0 (CK), 6 and 24 h after exposure to high temperature of 38 ℃, and low temperature of 4 ℃, and at 0 (CK), 6 and 12 h after UV-B stress.【Results】 Two Hsp90 genes were cloned from A. chinensis, and namely AcHsp83a (GenBank accession no.: OP791883) and AcHsp83b (GenBank accession no.: OP791884). Their open reading frames (ORFs) were 2 172 and 2 163 bp in length, encoding 723 and 720 amino acids with the relative molecular weight of 83.12 and 82.90 kD and the isoelectric point (pI) of 4.94 and 4.97, respectively, and the C-terminal sequences both contain the conserved motif EEVD, showing that they belong to the cytoplasmic Hsps. AcHsp83a and AcHsp83b are highly conserved. The expression level of AcHsp83a was the highest in eggs, whereas that of AcHsp83b was the highest in adults. The expression level of AcHsp83a was the highest in the testis of male adults, whereas that of AcHsp83b was the highest in the midgut of female adults. When the female adults were exposed to 38 ℃, 4 ℃ or UV-B, the expression levels of AcHsp83a and AcHsp83b increased firstly and then decreased with treatment time, reaching the peak at 6 h. For the male adults, the expression level of AcHsp83a increased firstly and then decreased with treatment time, and reached the peak at 6 h after exposure to 38 ℃ or UV-B. The expression level of AcHsp83a in male adults decreased firstly and then increased with treatment time, and reached the peak at 24 h after exposure to 4 ℃. Compared with the control group, 38 ℃, 4 ℃ or UV-B exposure significantly decreased the expression levels of AcHsp83b in male adults. 【Conclusion】 The differential expression of AcHsp83a and AcHsp83b in A. chinensis suggests that the two genes play important roles in the growth and development of A. chinensis and contribute to its tolerance to extreme temperatures and UV-B stress.
    Functional analysis of Bmttv in the wing development of the silkworm, Bombyx mori
    LIANG Yan, LI Jing-Wen, CHEN Yin-Lin, JIA Wen-Yi, XIA Qing-You, HUA Xiao-Ting
    2023, 66(11):  1435-1442.  doi:10.16380/j.kcxb.2023.11.002
    Abstract ( 140 )   PDF (6678KB) ( 171 )   PDF(mobile) (6678KB) ( 49 )     
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     【Aim】 To analyze the function of glycosyltransferase gene Bmttv of Bombyx mori in the wing development.【Methods】 The full-length sequence of the Bmttv CDS of B. mori was cloned by RACE and analyzed by bioinformatics. The expression levels of Bmttv in different developmental stages (5th instar larva, larva at the wandering stage, prepupa, pupa and adult) and different tissues (cuticle, wing disc, testis, Malpighian tubules, trachea, silk gland, head, hemolymph, fat body and midgut) of the 5th instar larva of B. mori were detected by qRT-PCR. The overexpression vector was constructed and transiently transfected into BmE cells. Subcellular localization of BmTTV was performed by immunofluorescence. qRT-PCR was used to detect the expression levels of BmHippo, Bmwg, BmDpp, Bmsotv and BmHh, which are the key genes in the wing development of B. mori. 【Results】The CDS sequence of Bmttvof B. moriwas successfully cloned and is 2 228 bp in full-length. BmTTV has two special structural domains, both of which are related to the synthesis of heparan sulfate proteoglycans (HSPGs), with about 45% amino acid sequence identity to TTVs of Drosophila melanogaster and Homo sapiens, and closer affinity to TTVs of lepidopteran insects. Bmttv was expressed at high levels from the pupal stage and in the midgut, testis and wing disc of the 5th instar larva. BmTTV is mainly localized in the cytoplasm of BmE cells, and BmHippo, which is associated with the wing development, was significantly down-regulated in the expression after the overexpression of Bmttv. 【Conclusion】BmTTV may play an important role in the wing development of B. mori by participating in Hippo signaling pathway, and the results of this study lay a foundation for further research.
    Expression profiling of the cytochrome P450 superfamily genes in different developmental stages, tissues and female adults at different periods before and after blood meal of Anopheles sinensis (Diptera: Culicidae) based on the transcriptomic data
    GUO Ying-Ao, CHEN Bin
    2023, 66(11):  1443-1450.  doi:10.16380/j.kcxb.2023.11.003
    Abstract ( 91 )   PDF (16581KB) ( 155 )   PDF(mobile) (16581KB) ( 20 )     
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    【Aim】In this study, the cytochrome P450 superfamily genes of Anopheles sinensis were used to analyze their expression pattern in different developmental stages, different adult tissues and female adult at different periods before and after blood meal of A. sinensis, so as to lay the foundation for further studies on the function of the cytochrome P450 superfamily genes.【Methods】 Samples were collected from A. sinensis laboratory deltamethrin-susceptible strain (WX-LS) at different developmental stages (egg, 1st-4th instar larvae, male pupa, female pupa, male adult and female adult), adult tissues (antenna, salivary gland, midgut, Malpighian tubules, testis, ovary, cuticle and fat body), and female adult at 1, 3, 6, 12, 24, and 48 h after blood meal to perform transcriptome sequencing using Illumina HiSeqTM2000. Based on the sequenced transcriptome data, the expression levels of cytochrome P450 genes in different developmental stages, different adult tissues and female adult before and after blood meal of A. sinensis were analyzed. 【Results】 The expression of the cytochrome P450 superfamily genes in A. sinensis was both developmental stage and tissue specific. In the larval, pupal and adult stages, 40, 26 and 35 genes were expressed at high levels, with CYP6AG1, CYP6AG2, CYP6AH1, CYP6Z3, CYP6Y2, CYP6P4, CYP9J3 and CYP9J4 being highly expressed throughout the larval stage. In A. sinensis adult, CYP6AA1, CYP6AH1, CYP6M4, CYP9J3, CYP9J4 and CYP12F3 were highly expressed in the midgut and Malpighian tubules, and CYP4G16, CYP6AA1, CYP6AH1, CYP6Y1, CYP6Z3, CYP9J3 and CYP9J4 were highly expressed in all adult tissues. CYP4G16, CYP6AA1, CYP6AG2, CYP6AH1, CYP6Y1, CYP6Y2, CYP9K1, CYP12F3 and CYP304B1 in female adult of A. sinensis were highly expressed at 1, 3, 6, 12, 24 and 48 h after blood meal. 【Conclusion】Cytochrome P450 superfamily genes highly expressed in the larval stage may be involved in the detoxification of food-borne toxic chemicals, and those highly expressed in the midgut and Malpighian tubules of female adult may play an important role in the detoxification process. These results lay a preliminary foundation for further investigation of the functions of cytochrome P450 superfamily genes in the growth and development, expression regulation and insecticide metabolism mechanism of A. sinensis.
    Construction of yeast two-hybrid cDNA library of Musca domestica (Diptera: Muscidae) and screening of MD14-3-3 interacting proteins
    CHEN Ming-Ming, JIAO Zhen-Long , ZHAO Wen-Jing, GUO Guo
    2023, 66(11):  1451-1458.  doi:10.16380/j.kcxb.2023.11.004
    Abstract ( 73 )   PDF (21480KB) ( 218 )   PDF(mobile) (21480KB) ( 18 )     
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    【Aim】To construct a yeast two-hybrid (Y2H) cDNA library of Musca domestica and investigate the proteins that could interact with the MD14-3-3. 【Methods】 Taking M. domestica as the research object, the mRNA was purified, the primary cDNA library was constructed, the secondary library was constructed by the recombinant pGADT7-DEST vector, so as to construct the Y2H. Then, the plasmid pGBKT7-MD14-3-3 were constructed as bait and used for screening the proteins interacting with MD14-3-3. Finally, the transformation and validation were verified. 【Results】 A Y2H cDNA library of M. domestica was successfully constructed, with the library storage capacity of 1.6×107 CFU and the recombination rate of 100%. Two target proteins were obtained by Y2H screening. The results of transformation and validation showed that mucin-like protein HKR1 and antibacterial peptide ctenidin-1 interacted with MD14-3-3.【Conclusion】 Two proteins that interact with MD14-3-3 were identified from the successfully constructed Y2H cDNA library of M. domestica. Our results provide a basis for investigating the mechanism of immune response of M. domestica.
    Molecular cloning and prokaryotic expression of VdesNPC2 protein in Varroa destructor (Acari: Varroidae) and the analysis of its binding mechanism to the host larval pheromones
    LIU Shen-Yun, WANG Jia-Li, YUAN Xing-Guang, WANG Cai-Die, TU Wan-Jun, ZHOU Wen-Run, LI Hong-Liang, WU Fan
    2023, 66(11):  1459-1466.  doi:10.16380/j.kcxb.2023.11.005
    Abstract ( 75 )   PDF (1806KB) ( 168 )   PDF(mobile) (1806KB) ( 15 )     
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    【Aim】To elucidate the function of Niemann-Pick type C2 protein of Varroa destructor (VdesNPC2b) in host recognition by analyzing the binding properties and mechanisms of VdesNPC2b with the larval pheromones methyl oleate and β-ocimene of the host bees of V. destructor, so as to provide a theoretical basis for biological control of V. destructor. 【Methods】 The open reading frame (ORF) of VdesNPC2b was amplified and analyzed using bioinformatics. The prokaryotic expression vector was constructed based on pET-30a plasmid. The recombinant VdesNPC2b protein was obtained by prokaryotic expression and affinity column chromatography. The binding capacities of VdesNPC2b with the larval pheromones of bees methyl oleate and β-ocimene were analyzed by fluorescence competitive binding experiment, and the binding mechanism of them was analyzed by measuring the binding capacity change at two different temperatures (22 and 32 ℃) through fluorescence spectrum temperature variation experiment. The homologous modeling of VdesNPC2b was performed by SWISS-MODEL software, and the molecular docking simulation of VdesNPC2b and β-ocimene was performed by MVD to preliminarily analyze the key amino acid sites in the binding of VdesNPC2b and β-ocimene. 【Results】 The ORF of VdesNPC2b (GenBank no.: OR463903) is 531 bp in full-length, encoding 176 amino acids. VdesNPC2b has a signal peptide of 16 amino acid residues at the N-terminus. The fluorescent competitive binding assay result showed that the dissociation constant KD values of VdesNPC2b with methyl oleate and βocimene were 2.89 and 3.49 μmol/L, respectively, with the binding process of dynamic quenching, and the main driving forces maintaining the interaction between VdesNPC2b and methyl oleate and β-ocimene was hydrophobic force. Homologous modeling showed that the secondary structure of VdesNPC2b is β-sheet, and forms a potential external cavity. Leu68, Ile103 and Phe107 could be the key amino acid sites to maintain a stable form of the binding of VdesNPC2b and β-ocimene. 【Conclusion】 V. destructor may use VdesNPC2b binding long-chain brood ester pheromone methyl oleate and volatile β-ocimene to locate and identify host honey bee.
    Effects of different diets on the growth and development, reproduction and the adult intestinal bacterial community of Dastarcus helophoroides (Coleoptera: Bothrideridae)
    GUO Jia-Li, ZHANG Cen-Yu, ZHANG Meng, TANG Guang-Hui, ZHANG Zheng-Qing
    2023, 66(11):  1467-1481.  doi:10.16380/j.kcxb.2023.11.006
    Abstract ( 35 )   PDF (2767KB) ( 165 )   PDF(mobile) (2767KB) ( 17 )     
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    【Aim】The artificial breeding and propagation of Dastarcus helophoroides have been successfully achieved indoors. However, different diet formulae have significant effects on the growth and development of D. helophoroides adults and their offspring. According to the artificial diets allocated by D. helophoroides adults under field conditions, whether they are suitable for the growth and development of long-term indoor breeding population at this stage needs to be further studied. The aim of this study is to investigate the effects of different diets on the growth and development, reproduction and the adult intestinal bacterial community of D. helophoroides, so as to provide a theoretical basis for optimizing diet formula.【Methods】Six different diet formulae [dry yeast 10 g+sucrose 3 g+agar 3 g+freeze-dried egg yolk powder 9 g+purified water 40 mL(Ⅰ), wood flour 5 g+dry yeast 10 g+sucrose 3 g+agar 3 g+freeze-dried egg yolk powder 9 g+purified water 35 mL (Ⅱ), silkworm pupa powder 10 g+dry yeast 10 g+sucrose 3 g+agar 3 g+freeze-dried egg yolk powder 9 g+purified water 30 mL(Ⅲ), dry powder of Zophobas atratus 10 g+dry yeast 10 g+sucrose 3 g+agar 3 g+freeze-dried egg yolk powder 9 g+purified water 30 mL(Ⅳ), wood flour 5 g+silkworm pupa powder 10 g+dry yeast 10 g+sucrose 3 g+agar 3 g+freeze-dried egg yolk powder 9 g+purified water 25 mL(Ⅴ) and wood flour 5 g+dry powder of Z. atratus 10 g+dry yeast 10 g+sucrose 3 g+agar 3 g+freeze-dried egg yolk powder 9 g+purified water 25 mL(Ⅵ)] were prepared and used to feed the newly emerged adult of D. helophoroides, using the newly emerged adult of D. helophoroides fed with no diet as the control group. Parameters including the adult body weight, body length, body width, cumulative mortality in 64 d, pre-oviposition period, average daily number of eggs laid per female and total number of eggs laid per female in 64 d of the parental generation were recorded. Additionally, parameters including the hatching rate, larval parasitism rate, cocooning rate, larval duration, cocoon period, and eclosion rate of the first-generation offspring of D. helophoroides were measured. At the same time, the Illumina NovaSeq was utilized to sequence the V4-V5 variation regions of 16S rDNA of the intestinal bacteria in the newly emerged adult of D. helophoroides fed with different diets. The diversity, species composition, and community structure differences of the intestinal bacteria were analyzed, and the gene function of the intestinal bacteria was predicted. The correlation analysis between the abundance of the core intestinal bacteria and various physiological parameters was conducted to elucidate the intestinal bacteria highly associated with the growth and development, and reproduction of D. helophoroides.【Results】 The body weight (0.0287 g) of D. helophoroides adults fed with diet Ⅵ was the highest. The total number of eggs laid per female in 64 d of D. helophoroides adults fed with diet Ⅵ was the highest (1 199.03 grains), followed by that of D. helophoroides adults fed with diet Ⅳ (1 068.19 grains), and those of D. helophoroides adults fed with dietsⅠ and Ⅲ were the lowest (756.11 and 732.61 grains, respectively). The body length (8.1319 cm) and cumulative mortality in 64 d (18.05%) of D. helophoroides adults fed with diets Ⅲ and Ⅱ, respectively, were the highest. Meanwhile, the pre-oviposition period of adult D. helophoroides fed with diets IV-VI was the shortest, averaging (21.44±0.20) d. The hatching rate and eclosion rate of the first-generation offspring of D. helophoroides from the adults fed with diet VI were the highest, and significantly higher than those from the adults fed with diets I and II. The larval parasitism rate (85.36%) and cocooning rate (97.34%) of the first-generation offspring of D. helophoroides from the adults fed with diets Ⅱ and Ⅲ, respectively, were the highest. A total of 4 781 552 optimized sequences of 16S rDNA were obtained from the intestinal bacteria of the newly emerged adults of D. helophoroides. A total of 758 operational taxonomic units (OTUs) were obtained by clustering analysis, and were annotated into 11 phyla, 22 classes, 40 orders, 63 families and 90 genera. At the phylum level, the intestinal dominant bacteria were Firmicutes and Protebacteria. At the genus level, the dominant intestinal bacteria of the newly emerged adults fed with diet was Lactococcus, with the abundance significantly increased compared to that of the control group. The relative abundance of the genus Lactococcus in the intestine of adults fed with diets IV-VI also gradually increased with feeding time. The results of alpha diversity analysis indicated that the intestinal bacterial diversity in the control group was the highest, while feeding on different diets resulted in a decreased intestinal bacterial diversity. Beta diversity analysis results showed that the adult intestinal bacterial community structure of the control group differed greatly from that fed with different diets. However, the intestinal bacterial community structure among adults fed with different diets was similar. KEGG analysis results revealed that the intestinal bacterial genes involved in metabolic pathways in adults fed with different diets at different time points were similar to those in the control group, with no significant differences. The abundance of core intestinal bacteria was correlated with adult growth, reproduction, and offspring larval adaptability. The presence of Pediococcus with higher relative abundance may reduce the hatching rate of the first-generation offspring, while Enterococcus may shorten the pre-oviposition period of the parent adults, reduce the larval parasitism rate and increase the eclosion rate of the first-generation offspring. However, Enterococcus had opposite effects in pre-oviposition period with Lactobacillus and in eclosion rate with Bacillus, respectively.【Conclusion】 Feeding on different diets significantly affects the adult growth and development, reproduction and intestinal microbes, and offspring fitness of D. helophoroides. Adults fed with artificial diet VI, which contained dry powder of Z. atratus and wood powder, exhibited the highest reproductive capacity and highest offspring fitness, indicating that this diet is more suitable for long-term indoor rearing of D. helophoroides populations. Concurrently, the intestinal bacterial underwent change in community structure as a result of feeding on different diets, leading to the decreased bacterial abundance and diversity but increased relative abundance of Lactococcus. Lactococcus may play an important role in the reproduction of adults and the growth and development of larvae, offering important references for improving the indoor breeding of D. helophoroides.
    Feeding preference and olfactory responses of Ostrinia furnacalis (Lepidoptera: Pyralidae) to Beauveria bassiana aerial conidia and blastospore-corn symbionts
    SUI Li, LU Yang, XIE Min, ZOU Xiao-Wei, LI Qi-Yun, ZHANG Zheng-Kun
    2023, 66(11):  1482-1489.  doi:10.16380/j.kcxb.2023.11.007
    Abstract ( 98 )   PDF (6578KB) ( 70 )   PDF(mobile) (6578KB) ( 10 )     
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    【Aim】 Beauveria bassiana can indirectly affect phytophagous insect pests by colonizing plants. This study aims to determine the responses of feeding preference and olfactory response of the neonate and 3rd instar larvae of Ostrinia furnacalis to symbionts of aerial conidia (AC) and blastospore (BS) B. bassiana-corn. 【Methods】 Each corn plant was inoculated with 20 mL B. bassiana spore suspension (1.0×108 spore/mL), soil drench inoculation was used to establish the B. bassiana-corn symbionts, including the B. bassiana AC treatment group and the B. bassiana BS treatment group. The feeding selection rates by the neonate larvae and 3rd instar larvae of O. furnacalis to the corn leaves of the B. bassiana-corn symbionts of AC and BS treatment groups under the choice and non-choice conditions were tested by a dish leaf disc method. The olfactory selection coefficient and olfactory selection response rate of the neonate and 3rd instar larvae of O. furnacalis to the corn leaves of the B. bassiana-corn symbionts of AC and BS treatment groups were detected by Y-type olfactometer. 【Results】 The test results of the dish leaf disc method showed that the feeding selection rate of both the neonate and 3rd instar larvae of O. furnacalis to the corn leaves of the B. bassiana-corn symbionts of AC and BS treatment groups were lower than that of the control group under the choice and non-choice conditions. The neonate larvae of O. furnacalis had the lowest feeding selection rate to the corn leaves of the B. bassiana-corn symbionts of BS treatment group under the choice condition, while the neonate larvae of O. furnacalis had no significant difference in the feeding selection rate to the corn leaves of the B. bassiana-corn symbionts of AC and BS treatment groups under the non-choice condition. The results of Y-type olfactometer test showed that the olfactory selection response rate of both the neonate and 3rd instar larvae of O. furnacalis to the corn leaves of the B. bassiana-corn symbionts in AC and BS treatment groups (olfactory selection coefficient<0) were significantly lower than that in the control group (olfactory selection coefficient>0), suggesting that the two types of B. bassiana spore colonized corn leaves have negative chemotaxis to O. furnacalis larvae. 【Conclusion】 This study confirms that there is an obvious avoidance effect of the corn leaves colonized by aerial conidia and blastospores of B. bassiana on the feeding behavior and olfactory behavior of O. furnacalis, which could provide a theoretical basis to utilize the endophytic colonization of B. bassiana for ecological control of insect pests.
    Response of heat inducible heat shock protein 90 gene of Aphis gossypii (Hemiptera: Aphididae) to gossypol and flupyradifurone stresses and mutual effect on transcription factor HSF (In English)
    LIANG Ping-Zhuo, LI Ren, CHENG Shen-Hang, LI Da-Peng, ZHANG Lei, ZHEN Cong-Ai
    2023, 66(11):  1490-1499.  doi:10.16380/j.kcxb.2023.11.008
    Abstract ( 70 )   PDF (1168KB) ( 221 )   PDF(mobile) (1168KB) ( 5 )     
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    【Aim】The aim of this study is to identify heat shock protein (Hsp) gene in Aphis gossypii(AgHsp90) and clarify the response of AgHsp90 to temperature, plant allelochemicals and insecticide stresses. 【Methods】RT-PCR and RACE were used to clone AgHsp90 of A. gossypii, and bioinformatics analysis was conducted. The expression levels of AgHsp90 in different developmental stages (1st-4th instar nymphs and adult) and in adults at 1 h after treatments with -5, 0, 5, 10, 15, 20, 30, 35, 38 and 42 ℃, and at 24 h after treatment with plant allelochemicals (20 mg/L tannic acid, 50 mg/L gossypol, 50 mg/L 2-tridecanone and 50 mg/L quercetin) and pesticide flupyradifurone stress[LC25(2.410 mg/L)] were quantified by qRT-PCR. RNAi of AgHsp90 and heat shock factor (HSF) gene AgHSF of A. gossypii adult was performed for 24 h through feeding method to determine the mortality rate of A. gossypii adults at 24 and 48 h after treatments with 50 mg/L gossypol and LC40 (4.649 mg/L) flupyradifurone through feeding and leaf dipping methods, respectively, explore the effect of AgHsp90 on the susceptibility of A. gossypii to gossypol and flupyradifurone and preliminarily verify the mutual regulation between AgHsp90 and AgHSF. 【Results】One Hsp90 gene of A. gossypii designated AgHsp90 (GenBank accession no.: UOF38310) was obtained with a 2 181 bp ORF in length. The expression levels of AgHsp90 were relatively stable in different developmental stages, with the significant difference happened between the 3rd instar nymph and adult. Temperature stress experiment result revealed that AgHsp90 was obviously high temperature inducible, but low temperature downregulated the expression level of AgHsp90. The expression level of AgHsp90 in A. gossypii adult was not significantly induced by three plant allelochemicals (gossypol, tannic acid, and quercetin) and significantly induced by 2-tridecanone and LC25 flupyradifurone compared with the controls 0.5 mol/L sucrose and Triton X-100, respectively. RNAi of AgHsp90 could remarkably increase the mortality rate of A. gossypii adult resulted from gossypol and flupyradifurone compared with the control fed with dsGFP. AgHsp90 and its transcription factor AgHSF could mutually affect the expression levels using RNAi method. 【Conclusion】 The heat shock protein gene AgHsp90 of A. gossypii is high temperature and flupyradifurone inducible. AgHsp90 is mostly associated with the gossypol and flupyradifurone susceptibility of A. gossypii. The above results indicate that AgHsp90 may play crucial roles in response to high temperature and pesticide flupyradifurone stresses.
    Sublethal effects of abamectin on the development and reproduction of Sogatella furcifera (Hemiptera: Delphacidae) (In English)
    LIAO Qi, ZHAO Bin-Bin, FANG Ling, ZHOU Nan-Xin, HE Shu-Lin, XU Shan, YANG Hong, ZHOU Cao
    2023, 66(11):  1500-1509.  doi:10.16380/j.kcxb.2023.11.009
    Abstract ( 91 )   PDF (1700KB) ( 185 )   PDF(mobile) (1700KB) ( 14 )     
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    【Aim】 To investigate the sublethal effects of abamectin on Sogatella furcifera. 【Methods】The 3rd instar nymphs of S. furcifera were treated with abamectin at LC10 (0.016 mg/L) and LC25 (0.031 mg/L) concentrations using rice seedling dipping method. The longevity of female and male adults and the number of eggs laid per female of F0 generation were recorded, and the developmental duration, fecundity, survival rate, female and male adult longevity, intrinsic rate of increase (r), finite rate of increase (λ), net reproductive rate (R0), and mean generation time (T) of F1 generation were recorded, and the age-stage, two-sex life table was established. 【Results】 After the 3rd instar nymphs of S. furcifera were exposed to LC10 and LC25 concentrations of abamectin, the number of eggs laid per female of the F0 generation was significantly reduced, but the hatching rate was not affected as compared to the control (distilled water). Abamectin at the LC25 concentration significantly prolonged the male adult longevity, whereas the female adult longevity was significantly shortened by the LC10 of abamectin compared with the control. In addition, LC10 and LC25 concentrations of abamectin significantly reduced the number of eggs laid per female of the F1 generation (LC10: 170.64 grains; LC25: 155.79 grains)  compared with the control (193.38 grains). In the LC10 and LC25 concentration treatment groups, the intrinsic rate of increase (r), finite rate of increase (λ) and mean generation time (T) of the F1 generation were slightly lower than those in the control group, while the reproductive rate (R0) showed a decreasing trend but not significantly different from that in the control. 【Conclusion】 Abamectin stress at the sublethal concentrations could somewhat inhibit the offspring population growth of S. furcifera.
    Effects of LED light on the activities of protective enzymes in Ectropis grisescens (Lepidoptera: Geometridae) adults
    QIAO Li, ZHAO Xiao-Cen, CHEN Lei, ZHOU Zhou, GENG Shu-Bao, WU Yu-Qing, ZHANG Fang-Mei
    2023, 66(11):  1510-1517.  doi:10.16380/j.kcxb.2023.11.010
    Abstract ( 61 )   PDF (1110KB) ( 161 )   PDF(mobile) (1110KB) ( 9 )     
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    【Aim】To study the effects of wavelength, intensity and treatment time of LED light on the activities of protective enzymes in Ectropis grisescens adults, so as to provide references for the green control of E. grisescens with light in tea garden.【Methods】The female and male adults of E. grisescens were exposed to LED light at the wavelengths of 590-595 and 520-525 nm under the light intensities of 40, 80 and 120 lx, respectively, in the laboratory, and the changes in the total protein content, activities of protective enzymes [peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT)], and total antioxidant capacity (T-AOC) were tested at 1, 2 and 3 h after treatments.【Results】 After exposure to LED light at the wavelengths of 590-595 and 520-525 nm, the total protein content, the activities of POD, SOD and CAT, and T-AOC in female and male adults of E. grisescens increased with the increase of light intensity and treatment time. At different time after exposure to LED light at the two wavelengths under various light intensities, the total protein content and POD activities in female adults were significantly higher than those in male adults, and the activities of SOD and CAT, and T-AOC in male adults were higher than those in female adults and those in female and male adults at 3 h under LED light intensity of 120 lx were the highest. The activities of POD, SOD and CAT in female and male adults of E. grisescens subjected to LED light radiation at the two wavelengths for different time was higher than those in the natural light control.【Conclusion】 LED light at the wavelengths of 590-595 and 520-525 nm can cause changes in the protective enzyme activities in E. grisescens adults, providing basic data for exploring the best wavelength, intensity and time of breaking protective enzyme activity and green control using light to irradiate pests in tea garden.
    Effects of irradiating the parasitized Drosophila melanogaster pupae with ultraviolet rays on the growth and development of Trichopria drosophilae (Hymenoptera: Diapriidae)
    LIU Xu-Xiang, YANG Yong-Bang, FAN Qing-Wen, ZHANG Qin-Yuan, JI Qing-E
    2023, 66(11):  1518-1526.  doi:10.16380/j.kcxb.2023.11.011
    Abstract ( 82 )   PDF (1512KB) ( 240 )   PDF(mobile) (1512KB) ( 9 )     
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    【Aim】To clarify the effects of ultraviolet radiation to the parasitized host pupa on the growth and development of the parasitoid Trichopria drosophilae, so as to provide a reference for the mass breeding of T. drosophilae. 【Methods】Three ultraviolet irradiation lamps, UVA, UVB and UVC, were used to irradiate the parasitized Drosophila melanogaster pupae in a dark box. The irradiation duration was 3, 6 and 9 h, respectively. The pupal mortality rate, egg-adult duration, adult emergence rate, sex ratio (female to male ratio), and longevity of T. drosophilae were observed and calculated. 【Results】Compared to the control, UVA irradiation showed no significant effects on the adult emergence rate, pupal mortality rate and female to male ratio of T. drosophilae. After UVB irradiation for 3, 6 and 9 h, and UVC irradiation for 3 and 9 h, the adult emergence rates of T. drosophilae decreased significantly and the pupal mortality rates increased significantly compared to the control. The female to male ratios of T. drosophilae irradiated by UVB for 3, 6 and 9 h were greater than 2.00. After UVA irradiation for 3 and 6 h, and UVC irradiation for 3, 6 and 9 h, the egg-female adult duration of T. drosophilae was significantly lower than that of the control. After UVC irradiation for 3, 6 and 9 h, the egg-male adult duration of T. drosophilae was significantly lower than that of the control. The female adult longevity of T. drosophilae raised with the increase of irradiation duration of UVA, but showed no significant difference between the UVA irradiation groups and the control. The female adult longevity in UVB irradiation for 6 h was significantly higher than that in the control. The male adult longevity of T. drosophilae in ultraviolet irradiation treatments was higher than that of the control, and the male adult longevity in UVA irradiation for 3 h, UVB irradiation for 6 h and UVC irradiation for 9 h exhibited significant difference from that in the control. 【Conclusion】Ultraviolet irradiation treatment on the parasitized D. melanogaster pupae can significantly affect the egg-adult duration and adult longevity of T. drosophilae. The results of this study provide a reference for the multiplication of natural enemy resources for biological control of Drosophila pests.
    REVIEW ARTICLES
    Research progress on senescence and longevity regulation of honeybees
    JIN Meng-Jie, ZENG Zhi-Jiang
    2023, 66(11):  1527-1534.  doi:10.16380/j.kcxb.2023.11.012
    Abstract ( 127 )   PDF (1061KB) ( 295 )   PDF(mobile) (1061KB) ( 21 )     
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     Honeybee queens share the same genetic background with the workers, and they both are developed from fertilized eggs. Nutritional and spatial variances during the development lead to significant morphological, physiological and behavioral dimorphism between the two castes. The insulin signaling pathway (IIS) regulates the behavior of worker bees, thus influencing their longevity. Longer longevity of queen bees is associated with increased oxidative stress and enhanced stress defense. Vitellogenin (Vg) interacts with juvenile hormone (JH), and the relatively high level of Vg and low level of JH usually lead to longer longevity. Telomerase activity and telomere length are influenced by the development and caste of honeybees. Queens inherit longer telomere length and maintain higher telomerase activity than workers. Overwintering worker bees live longer and show higher telomerase activity than the summer worker bees. Mitochondrial damage is a sign of senescence, while the mitochondrial function of the aged queen remains vigorous. Senescence is closely related to DNA methylation, and DNA methylation and histone modification play important roles in the regulation of plasticity in social insects. With the increase in population aging and the high prevalence of senescence-related diseases, “healthy aging” has triggered a series of concerns in life sciences and social sciences. Studies on honeybee senescence and longevity regulation will provide an important reference for the biology of senescence.
    SHORT COMMUNICATIONS
    Release patterns and field verification of 2-ethyl-3,6-dimethylpyrazine, the alarm pheromone of Solenopsis invicta (Hymenoptera: Formicidae), in natural rubber plug and PVC pipe carriers
    DING Hao-Yi, HUANG Jun, ZHANG Juan, LI Xiao-Wei, ZHANG Da-Yu, LÜ Yao-Bin
    2023, 66(11):  1535-1542.  doi:10.16380/j.kcxb.2023.11.013
    Abstract ( 78 )   PDF (1358KB) ( 104 )   PDF(mobile) (1358KB) ( 10 )     
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     【Aim】To clarify the release patterns of 2-ethyl-3,6-dimethylpyrazine, the alarm pheromone of the red imported fire ant, Solenopsis invicta in two different slow-release carriers, the natural rubber plug (NRP) carrier and the PVC pipe (PVCP) carrier, and to verify the results in the field test. 【Methods】A standard curve of the relationship between the dose and peak area of 2-ethyl-3,6dimethylpyrazine was established by using headspace solid-phase microextraction (HS-SPME) and gas chromatogram-mass spectrometry (GC-MS). The release amount and release rates of 2-ethyl-3, 6-dimethylpyrazine at different doses were determined after extraction in these two slowrelease carriers for 15, 30 and 60 min, respectively. 【Results】In NRP carriers, the release rate of 2-ethyl-3, 6-dimethylpyrazine at the same dose gradually slowed down with the increase of extraction time. There were significant differences in the release amount of 2-ethyl-3, 6-dimethylpyrazine at the dose of 10 000 ng between 15 and 30 min, and between 15 and 60 min extraction, and in the release amount of 2-ethyl-3,6-dimethylpyrazine at the dose of 100 000 ng between 60 and 15 min, and between 60 and 30 min extraction. There were significant differences in the release dynamics of 2-ethyl-3, 6-dimethylpyrazine at the same dose among different extraction time. In PVCP carriers, the release rate of 2-ethyl-3, 6-dimethylpyrazine at the same dose was gradually increased with the extraction time. There were significant differences in the release amount of 2-ethyl-3, 6-dimethylpyrazine at the same dose among different extraction time. In the field test, we confirmed that 1 000 ng of 2-ethyl-3, 6-dimethylpyrazine in NPR carriers significantly attracted S. invicta, and with the increase of dose, 2-ethyl-3, 6-dimethylpyrazine showed a significant repellent effect against S. invicta. In PVCP carriers, 10 000 ng of 2-ethyl-3,6-dimethylpyrazine had a significant attractant effect on S. invicta, and with the increase of the dose, 2-ethyl-3, 6-dimethylpyrazine showed a significant repellent effect against S. invicta. 【Conclusion】The release rate of 2-ethyl-3,6-dimethylpyrazine, the alarm pheromone of S. invicta in NRP carriers gradually slows down with the increase of extraction time, while its release rate in PVCP carriers gradually speeds up with the extraction time. Also, 1 000 ng of 2-ethyl-3, 6-dimethylpyrazine used with NRP carriers or 10 000 ng of 2-ethyl-3,6-dimethylpyrazine used with PVCP carriers can be used for the monitoring of S. invicta, and the NRP carrier is more suitable for the rapid detection of S. invicta.
    CONTENTS
    Contents of Vol. 66 Issue 11
    2023, 66(11):  1543-1543. 
    Abstract ( 37 )   PDF (359KB) ( 247 )     
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