›› 2005, Vol. 48 ›› Issue (3): 444-449.

• 研究论文 • 上一篇    下一篇

棉铃虫幼虫中肠非糖基磷酯酰肌醇锚着氨肽酶N基因的克隆和测序

苏建亚, 沈晋良*   

  1. 南京农业大学农药科学系
  • 出版日期:2005-07-12 发布日期:2005-06-20
  • 通讯作者: 沈晋良

Cloning and sequencing of midgut aminopeptidase N without glycosylphosphatidylinositol (GPI) anchor from larvae of Helicoverpa armigera(Hübner)

SU Jian-Ya, SHEN Jin-Liang*   

  1. Nanjing Agricultural University
  • Online:2005-07-12 Published:2005-06-20

摘要:

通过对棉铃虫Helicoverpa armigera (Hübner)幼虫中肠氨肽酶N的克隆和测序,鉴定了1个氨肽酶N基因APN1,其cDNA序列具有3 220个核苷酸,具有3 042 bp的开放阅读框,编码产生1 014个氨基酸的蛋白质。其推定的氨基酸序列具有氨肽酶N所共有的锌结合模体HEXXHX18E和N末端20个氨基酸的疏水性信号序列,但C末端没有糖基磷酯酰肌醇(glycosylphosphatidylinositol,GPI)锚添加信号序列。该氨肽酶N的cDNA序列已提交GenBank,登录号为AY358034。

关键词: 棉铃虫, 氨肽酶N, 糖基磷酯酰肌醇锚, 受体

Abstract:

cDNAs of aminopeptidase N (APN) from Helicoverpa armigera (Hübner) were cloned and sequenced. One aminopeptidase N (APN1) was identified. This aminopeptidase N had 3 220 nucleotides with a 3 042 bp open reading frame that encodes 1 014 amino acids. The putative amino acid sequence shares the consensus zinc binding/gluzincin motif HEXXHX18E and a hydrophobic signal sequence with 20 amino acids in Nterminal region common to all aminopeptidases, but no glycosylphosphatidylinositol (GPI) anchor signal sequence in Cterminal region of APN1 was found. The sequences reported here had been deposited in the GenBank database (Accession number: AY358034).

Key words: Helicoverpa armigera, aminopeptidase N, GPI anchor, receptor