昆虫学报 ›› 2020, Vol. 63 ›› Issue (9): 1125-1135.doi: 10.16380/j.kcxb.2020.09.010

• 研究论文 • 上一篇    下一篇

斑衣蜡蝉雌性生殖系统超微结构

刘佳宁, 秦道正*   

  1. (西北农林科技大学植物保护学院, 陕西杨凌 712100)
  • 出版日期:2020-09-20 发布日期:2020-09-30

Ultrastructure of the female reproductive system of Lycorma delicatula (Hemiptera: Fulgoridae)

LIU Jia-Ning, QIN Dao-Zheng*   

  1. (College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China)
  • Online:2020-09-20 Published:2020-09-30

摘要: 【目的】明确斑衣蜡蝉Lycorma delicatula雌成虫生殖系统整体形态及超微结构特征,为蜡蝉总科昆虫分类及系统发育探讨提供更多形态学证据。【方法】采用光学显微镜与透射电子显微镜,观察斑衣蜡蝉雌成虫生殖系统整体形态和各主要器官的超微结构。【结果】斑衣蜡蝉雌成虫生殖系统主要包括1对卵巢、1个中输卵管、1个交配囊、1个交配囊管、1个前阴道、1个后阴道、1个受精囊、1个受精管和2根受精囊附腺。卵巢为端滋式,由14根卵巢小管组成,卵室由固有膜、滤泡细胞和卵细胞组成,卵巢小管中的滋养细胞清晰可见;中输卵管位于前阴道基部,由中输卵管腔、上皮细胞、肌肉鞘和基膜组成;交配囊膨大呈圆球状,囊壁由上皮细胞、肌肉层和基膜组成;交配囊管呈圆柱状,连接交配囊和后阴道,由肌肉鞘、上皮细胞层和管腔组成;前、后阴道超微结构相似,主要由肌肉鞘、基膜、上皮细胞和管腔组成,但后阴道上皮细胞细胞核周围存在分泌颗粒,且管腔内有大量微绒毛,而前阴道壁内包含有大量囊泡结构;受精管从中输卵管末端延伸至受精囊,由基膜、厚层肌肉鞘和管腔组成;受精囊为受精管近末端略膨大的囊状结构,由肌肉鞘、基膜、上皮细胞和囊腔构成;雌性受精囊附腺着生于受精囊末端,为均匀的螺旋管状,主要由肌肉层、上皮细胞层和附腺中心管腔组成。【结论】斑衣蜡蝉雌性生殖系统与已报道的蜡蝉总科其他类群的雌性生殖系统结构相似,但卵巢小管数目有差异;蝉亚目中不同总科雌成虫雌性附腺与受精囊附腺的形态特征存在明显区别;斑衣蜡蝉雌性生殖系统超微结构与叶蝉总科和沫蝉总科昆虫也存在部分差异。这些差异是否可以作为头喙亚目高级阶元的划分依据仍有待于进一步研究。

关键词: 蜡蝉科, 斑衣蜡蝉, 雌性生殖系统, 形态, 超微结构, 透射电子显微镜

Abstract: 【Aim】 This study aims to clarify the whole morphology and ultrastructure characteristics of the female reproductive system of the spotted lanternfly, Lycorma delicatula, so as to provide more morphological evidence for the taxonomy and phylogenetic analysis of the superfamily Fulgoroidea. 【Methods】 The general morphology and ultrastructure of the reproductive system of female adult of L. delicutula were observed by light microscope and transmission electron microscope. 【Results】 The reproductive system of female adult of L. delicatula mainly includes one pair of ovaries, one common oviduct, one bursa copulatrix, one bursa copulatrix duct, one anterior vagina, one posterior vagina, one spermatheca, one ductus receptaculi and two spermathecal accessory glands. The ovary is telotrophic and consists of 14 ovarian tubules, the egg chamber is composed of tunica propria, follicular cells and oocytes, and trophoblasts in ovarioles are clearly visible. The common oviduct is located at the ventral part of the anterior vagina, and comprised of lumen, epithelial cells, muscle sheath and basement membrane. The bursa copulatrix is spherical, and the wall is composed of epithelial cells, muscle layer and basement membrane. The bursa copulatrix duct is cylindrical, connecting with bursa copulatrix and posterior vagina, and comprised of muscle sheath, epithelial cell and lumen. The ultrastructure of the anterior and posterior vagina is similar, mainly composed of muscle sheath, basement membrane, epithelial cells and lumen, but there are secretory granules around the nucleus of the posterior vagina epithelial cells and lots of microvilli in the lumen, while there are lots of vesicles in the anterior vagina wall. The ductus receptaculi extends from the end of common oviduct to spermatheca and consists of basement membrane, thick muscular sheath and lumen. The spermatheca is saclike and slightly enlarged at the proximal end of the ductus receptaculi, and composed of muscle sheath, basement membrane, epithelial cells and lumen. The female spermathecal accessory gland is located at the end of the spermatheca, and in the from of a uniform spiral tube mainly composed of muscle layer, epithelial cell layer and the central lumen of the accessory gland. 【Conclusion】 The reproductive system of female adult of L. delicutula is similar to those of the previously reported fulgoroid groups, but the number of ovarian tubules differs from those of other families. The morphological characteristics of female accessory glands and spermathecal gland differ among families in the suborder Auchenorrhyncha. The ultrastructure of L. delicatula is also somewhat different from that of the species in Cicadelloidea and Cercopoidea. Whether these differences can be used for the division of higher taxa of Auchenorrhyncha needs to be studied further.

Key words: Fulgoridae; Lycorma delicatula, female reproductive system, morphology; ultrastructure, transmission electron microscope