Abstract: 【Aim】 To elucidate the phylogeny of subfamilies within the family Coreidae by analyzing the mitochondrial genome sequence data. 【Methods】 High-throughput sequencing was used to conduct low-coverage whole-genome sequencing of four coreid species (Homoeocerus striicornis, H. dilatatus, Cletus schmidti and Molipteryx fuliginosa). The complete mitochondrial genome sequences were reconstructed from the obtained whole-genome sequencing data. Combining with the published mitochondrial genome sequences of 32 species from three subfamilies of Coreidae (as ingroups) and three mitochondrial genome sequences from the family Alydidae (as outgroups), the phylogeny of subfamilies within Coreidae was reconstructed using both the maximum likelihood and Bayesian inference methods. 【Results】 The lengths of the mitochondrial genomes of H. striicornis, H. dilatatus, C. schmidti and M. fuliginosa (GenBank accession numbers: OR702557-OR702560) were 15 706, 15 913, 17 685 and 16 959 bp, respectively. The gene arrangements of the newly obtained mitochondrial genomes of the four coreid species were consistent with that of the typical insect mitochondrial genome, showing no gene rearrangement or other special genomic structural characteristics. Furthermore, the complete mitochondrial genome sequences, and the protein-coding gene, rRNA gene and tRNA gene sequences of the mitochondrial genomes of the four coreid species exhibited high A+T content (≥70%). The phylogenetic trees of Coreidae reconstructed using the maximum likelihood and Bayesian inference methods had a largely identical tree topology, supporting Coreinae as a monophyletic and a sister-group relationship between Coreinae and Hydarinae. 【Conclusion】 This study reconstructed the phylogeny of subfamilies within Coreidae using mitochondrial genome data. The results support the monophyletic group of the subfamily Coreinae and the sister relationship between Coreinae and Hydarinae. This study provides mitochondrial phylogenomic data for further exploration of the systematic evolution of Coreidae in a phylogenetic framework.

Key words:  Hemiptera, Coreoidea, Coreidae, mitochondrial genome, phylogeny